PXD053042

PXD053042 is an original dataset announced via ProteomeXchange.

Title	Full proteome analysis of Hoxb8 immortalized murine myeloid progenitors knockout for CCDC134 and differentiated into macrophages
Description	We recently identified CCDC134 as a novel regulator of TLR responses. To gain a comprehensive understanding of the effects of CCDC134 loss, we conducted total proteome analysis on control sgRen and sgCCDC134 Hoxb8 immortalized murine myeloid progenitors that were differentiated into macrophages. Interestingly, we observed that plasma membrane and endolysosomal TLRs, along with their associated chaperone Gp96, were among the most significantly downregulated proteins. Our findings further demonstrated that CCDC134 is essential for the proper folding and stability of Gp96. As a result, the deletion of CCDC134 in various cell lines and Hoxb8-derived macrophages results in altered TLR folding and trafficking.
HostingRepository	PRIDE
AnnounceDate	2024-11-14
AnnouncementXML	Submission_2024-11-14_07:33:36.702.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Manuele Rebsamen
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-12 07:23:28	ID requested
⏵ 1	2024-11-14 07:33:37	announced

10.1084/JEM.20240825;

submitter keyword: CCDC134, Full proteome, Hoxb8 macrophages

Rebsamen Manuele
contact affiliation	Department of Immunobiology, University of Lausanne (155 chemin des Boveresses 1066 Epalinges Switzerland)
contact email	manuele.rebsamen@unil.ch
lab head
Manuele Rebsamen
contact affiliation	Department of Immunobiology, University of Lausanne, Switzerland
contact email	manuele.rebsamen@unil.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/11/PXD053042

PRIDE project URI

• PRIDE
  1. PXD053042
     1. Label: PRIDE project
     2. Name: Full proteome analysis of Hoxb8 immortalized murine myeloid progenitors knockout for CCDC134 and differentiated into macrophages