PXD053023 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Electrophoresis-Correlative Framing of Ion Mobility Deepens Single-Cell Mass Spectrometry Proteomics |
Description | This project developed electrophoresis-correlative (Eco) mass spectrometry (MS) to measure the proteome of single cells on a trapped ion mobility mass spectrometer (timsTOF PRO). Eco-MS identified 962 protein groups in a <20 min of electrophoresing a single-HeLa-cell-equivalent (~200 pg) proteome amount. Match-between-runs improved this number to 2,139 protein groups on the same dataset. We demonstrate the approach to measure the proteome of single cells in X. laevis embryos at stage 8 (blastula). Single cells were isolated from the animal cap and their proteomes digested on a fluorosilane-coated microplate. Less than 4% of the available single-cell proteome was analyzed on a custom-built capillary electrophoresis (CE) platform under classical and Eco-driven ddaPASEF. Eco-MS returned ~51% of more protein groups than the control, identifying 1,157 proteins from N = 9 different single cells (biological replicates) of the animal cap. Eco-MS deepens the detectable coverage of the single-cell proteome using ddaPASEF on a timsTOF MS. |
HostingRepository | PRIDE |
AnnounceDate | 2025-02-06 |
AnnouncementXML | Submission_2025-02-06_07:44:14.529.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Peter Nemes |
SpeciesList | scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: 8355; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | timsTOF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-06-11 12:16:32 | ID requested | |
⏵ 1 | 2025-02-06 07:44:14 | announced | |
Publication List
Keyword List
submitter keyword: proteomics, Xenopus laevis, mass spectrometry,Single cell, capillary electrophoresis |
Contact List
Peter Nemes |
contact affiliation | Department of Chemistry & Biochemistry, University of Maryland at College Park |
contact email | nemes@umd.edu |
lab head | |
Peter Nemes |
contact affiliation | University of Maryland, College Park |
contact email | nemes@umd.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/02/PXD053023 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD053023
- Label: PRIDE project
- Name: Electrophoresis-Correlative Framing of Ion Mobility Deepens Single-Cell Mass Spectrometry Proteomics