PXD053015

PXD053015 is an original dataset announced via ProteomeXchange.

Title	Cellular Resiliency and Survival of a Neuro-2a Cell Line Under Extreme Stress
Description	Stressors such as hypoxia, hypothermia, and acute toxicity often result in widespread cell death. Recently, the failure of a cryostorage dewar in our lab resulted in prolonged thawing of Neuro-2a cells, exposing them to a combination of these insults. Surprisingly, a small fraction of the cells survived the event, underwent a period of near-dormancy, and were able to fully recover to a healthy state. To investigate this unusual resiliency, we established a novel standardized model to recapitulate the event and charted the phenotypes, transcriptomes, proteomes, and mitochondrial dynamics of the surviving cells along the trajectory of recovery. We observed an enrichment in DNA-repair and chromatin modification pathways bolstered by highly elevated mitochondrial activity in the immediate aftermath of the event and through the early stages of recovery, with subsequent normalization toward that of controls as the cells completed their recovery. Our results indicate that a robust energetic state that helps sustain DNA repair and stress-responsive pathways at the earliest stages of recovery facilitate cell survival and resiliency following extreme stress.
HostingRepository	PRIDE
AnnounceDate	2024-11-26
AnnouncementXML	Submission_2024-11-26_10:00:20.508.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stanley Stevens
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-11 10:03:53	ID requested
⏵ 1	2024-11-26 10:00:21	announced

Hernandez-Jimenez R, Patel A, Machado-Olavarria A, Mathieu H, Wohlfahrt J, Guergues J, Stevens SM, Dharap A, Cellular resiliency and survival of Neuro-2a cells under extreme stress. Exp Cell Res, 443(1):114275(2024) [pubmed]

10.1016/j.yexcr.2024.114275;

submitter keyword: cryostorage, temperature, thawing, resiliency, stress, survival,cell, dewar

Dr. Stanley Stevens
contact affiliation	Deptartment of MBS, University of South Florida
contact email	smstevens@usf.edu
lab head
Stanley Stevens
contact affiliation	University of South Florida
contact email	smstevens@usf.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/11/PXD053015

PRIDE project URI

• PRIDE
  1. PXD053015
     1. Label: PRIDE project
     2. Name: Cellular Resiliency and Survival of a Neuro-2a Cell Line Under Extreme Stress