PXD053008

PXD053008 is an original dataset announced via ProteomeXchange.

Title	Identification of potential CCDC134 interactors by immunoprecipitation and LC-MS analysis
Description	Our data indicate that CCDC134 plays a critical role in regulating the biogenesis of Toll-like receptors (TLRs). To gain deeper insights into the molecular mechanisms through which CCDC134 mediates TLR regulation, we sought to identify potential binding partners using interaction proteomics. Mass spectrometry analysis of Flag-CCDC134 immunoprecipitates from stably reconstituted CCDC134 knockout CAL-1 cells provided a better understanding of the interaction landscape associated with CCDC134. Notably, among the identified proteins, the TLR chaperone Gp96 emerged as one of the strongest binding partners. We further validated that CCDC134 interacts with Gp96 and is essential for its proper folding and stability. Consequently, loss of CCDC134 impairs the folding, maturation, and trafficking of TLRs.
HostingRepository	PRIDE
AnnounceDate	2024-11-14
AnnouncementXML	Submission_2024-11-14_07:05:02.089.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Manuele Rebsamen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-11 06:50:45	ID requested
⏵ 1	2024-11-14 07:05:02	announced

10.1084/JEM.20240825;

submitter keyword: CCDC134, immunoprecipitation, CAL-1

Rebsamen Manuele
contact affiliation	Department of Immunobiology, University of Lausanne (155 chemin des Boveresses 1066 Epalinges Switzerland)
contact email	manuele.rebsamen@unil.ch
lab head
Manuele Rebsamen
contact affiliation	Department of Immunobiology, University of Lausanne, Switzerland
contact email	manuele.rebsamen@unil.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/11/PXD053008

PRIDE project URI

• PRIDE
  1. PXD053008
     1. Label: PRIDE project
     2. Name: Identification of potential CCDC134 interactors by immunoprecipitation and LC-MS analysis