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PXD053005

PXD053005 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleModulation of biological activities in adipose derived stem cells by histone deacetylation
DescriptionEpigenetic pharmaceuticals hold the promise of using cell-based regeneration to revolutionize wound healing treatment. Histone acetylation and deacetylation conducts an intriguing way that influences the fate of stem cells. In this context, we used pharmacological manipulation to coordinate histone acetylation in stem cells. When HDACs expression reduced or silenced using HDAC inhibitors, such as Suberoylanilide hydroxamic acid (SAHA), a spectacular transformation occurred at the cellular and the molecular levels. In our study, we used adipose-derived stem cells which received consecutive SAHA treatment for 72 hours. No change in the cell viability could be noticed at the studied concentrations. On the other hand, a decrease in the protein expression of histone deacetylases at 1000 nM was found. The cell proliferation marker (Ki-67) was increased after SAHA treatment compared to the vehicle as shown by immunofluorescence staining. The expression of CCND1 gene expression was upregulated, while the protein expression of the proliferating cell nuclear antigen was downregulated. Alternatively, microarray analysis revealed that CDKN1A and MDM2 genes were upregulated under the influence of SAHA. On the other hand, the mass spectrometry pathway enrichment analysis showed that cell cycle, ATP-dependent chromatin remodeling and DNA are among the regulated pathways. This study suggested that SAHA as pharmaco-epigenetic agent might alter ADSCs determination through coordinated biological processes.
HostingRepositoryPRIDE
AnnounceDate2025-05-07
AnnouncementXMLSubmission_2025-05-06_19:10:16.161.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSallam Abdallah
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListacetylated residue
InstrumenttimsTOF Pro
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-06-11 05:08:51ID requested
12025-05-06 19:10:17announced
Publication List
10.1038/s41598-024-84652-1;
Abdallah S, Tabebi M, Qanadilo S, Ali N, Wang J, D'Arcy P, Zhong W, Sjoberg F, Elmasry M, El-Serafi A, Modulation of biological activities in adipose derived stem cells by histone deacetylation. Sci Rep, 15(1):3629(2025) [pubmed]
Keyword List
submitter keyword: Histone deacetylase inhibitor, Suberoylanilide hydroxamic acid, differentiation, adipose derived stem cells, epigenetic, epigenetic modifier, Vorinostat
Contact List
Sallam A bdallah
contact affiliationPhD student, Linköping University
contact emailsallam.abdallah@liu.se
lab head
Sallam Abdallah
contact affiliationBKV linköping University
contact emailsallam.abdallah@liu.se
dataset submitter
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Dataset FTP location
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