⮝ Full datasets listing
PXD052996
PXD052996 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomic investigation of seed development under high nitrate supply in plants lacking Pyrroline-5-carboxylate dehydrogenase |
| Description | Free proline is an amino acid known to have many functions in plants, particularly in stress adaptation and development. Its metabolism is well known, but its exact role in plant physiology has remained elusive. Proline catabolism is ensured by the sequential action of the enzymes Proline dehydrogenase (ProDH) and Pyrroline-5-carboxylate dehydrogenase (P5CDH). In this study, seed development in the p5cdh mutant was investigated under low and high nitrate supply. It was found that, in p5cdh mutants, embryo development was blocked at the transition between embryogenesis and the maturation phase. As a result, the whole maturation phase was disturbed, starting with a shorter photosynthetic phase, followed by a lower accumulation of storage compounds but a higher accumulation of proline when compared to wild-type embryos. Finally, at the end of seed development, mutant embryos appeared to be unable to tolerate desiccation and died during this final stage of development. This phenotype was strongly enhanced under high nitrate. Together with multiomics analysis, these data show that proline catabolism is important for redox homeostasis and energy supply during seed maturation. This dataset contains the respective proteomic data of the project. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-01-14 |
| AnnouncementXML | Submission_2026-01-14_06:05:27.325.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Nils Rugen |
| SpeciesList | scientific name: Arabidopsis thaliana; common name: thale cress; NCBI TaxID: 3702; |
| ModificationList | Oxidation; Acetyl; Carbamidomethyl |
| Instrument | timsTOF Pro |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-06-10 23:48:55 | ID requested | |
| ⏵ 1 | 2026-01-14 06:05:28 | announced |
Publication List
| Dourmap C, Baud S, Eubel H, Rugen N, Crilat É, Beradocco S, Marnet N, Cl, é, ment G, Tcherkez G, Planchais S, Cabassa C, Lebreton S, Bordenave-Jacquemin M, Maldiney R, Carol P, Braun HP, Masclaux-Daubresse C, Bouchereau A, Savour, é A, Guivarc'h A, Pyrroline-5-carboxylate dehydrogenase is a key actor of nitrogen metabolism in maturing seeds of Arabidopsis thaliana. J Exp Bot, ():(2026) [pubmed] |
Keyword List
| submitter keyword: Arabidopsis, Seeds, Seed development, Pyrroline-5-carboxylate dehydrogenase , high nitrate supply |
Contact List
| Nils Rugen | |
|---|---|
| contact affiliation | Leibniz University Hannover |
| contact email | rugen@genetik.uni-hannover.de |
| lab head | |
| Hans-Peter Braun | |
| contact affiliation | Plant Genetics, department plant proteomics |
| contact email | braun@genetik.uni-hannover.de |
| lab head | |
| Nils Rugen | |
| contact affiliation | Leibniz University Hannover |
| contact email | rugen@genetik.uni-hannover.de |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v08/MSV000094979/ |
