PXD052983
PXD052983 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Alanine and glutamate catabolism collaborate to ensure the success of sporulation in Bacillus subtilis |
Description | Sporulation as a typical bacterial differentiation process has been studied for decades. However, two crucial aspects of sporulation, (i) the energy sources supporting the process, and (ii) the maintenance of spore dormancy throughout sporulation, are scarcely explored. Here, we reported the crucial role of RocG-mediated glutamate catabolism in regulating mother cell lysis, a critical step for successful sporulation of Bacillus subtilis, likely by providing energy metabolite ATP. Notably, rocG overexpression resulted in an excessive ATP accumulation in sporulating cells, leading to adverse effects on future spore properties, e.g. increased germination efficiency, reduced DPA content, and lowered heat resistance. Additionally, we revealed that Ald-mediated alanine metabolism was highly related to the inhibition of premature germination and the maintenance of spore dormancy during sporulation, which might be achieved by decreasing the typical germinant L-alanine concentration in sporulating environment. Our data inferred that sporulation of B. subtilis was a highly orchestrated biological process requiring a delicate balance in diverse metabolic pathways, hence ensuring both the completion of sporulation and production of high-quality spores. |
HostingRepository | iProX |
AnnounceDate | 2024-06-07 |
AnnouncementXML | Submission_2024-07-15_21:08:37.959.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Lei Rao |
SpeciesList | scientific name: Bacillus subtilis PY79; NCBI TaxID: 1415167; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2024-06-10 19:33:59 | ID requested | |
1 | 2024-07-10 21:22:14 | announced | |
⏵ 2 | 2024-07-15 21:08:38 | announced | 2024-07-16: Update information. |
Publication List
Lyu F, Yang D, Rao L, Liao X, Alanine and glutamate catabolism collaborate to ensure the success of Bacillus subtilis sporulation. Microbiol Res, 286():127828(2024) [pubmed] |
Keyword List
submitter keyword: TMT, Bacillus subtilis |
Contact List
Xiaojun Liao | |
---|---|
contact affiliation | China Agricultural University |
contact email | liaoxjun@cau.edu.cn |
lab head | |
Lei Rao | |
contact affiliation | China Agricultural University |
contact email | rao.lei@cau.edu.cn |
dataset submitter |
Full Dataset Link List
iProX dataset URI |