PXD052983

PXD052983 is an original dataset announced via ProteomeXchange.

Title	Alanine and glutamate catabolism collaborate to ensure the success of sporulation in Bacillus subtilis
Description	Sporulation as a typical bacterial differentiation process has been studied for decades. However, two crucial aspects of sporulation, (i) the energy sources supporting the process, and (ii) the maintenance of spore dormancy throughout sporulation, are scarcely explored. Here, we reported the crucial role of RocG-mediated glutamate catabolism in regulating mother cell lysis, a critical step for successful sporulation of Bacillus subtilis, likely by providing energy metabolite ATP. Notably, rocG overexpression resulted in an excessive ATP accumulation in sporulating cells, leading to adverse effects on future spore properties, e.g. increased germination efficiency, reduced DPA content, and lowered heat resistance. Additionally, we revealed that Ald-mediated alanine metabolism was highly related to the inhibition of premature germination and the maintenance of spore dormancy during sporulation, which might be achieved by decreasing the typical germinant L-alanine concentration in sporulating environment. Our data inferred that sporulation of B. subtilis was a highly orchestrated biological process requiring a delicate balance in diverse metabolic pathways, hence ensuring both the completion of sporulation and production of high-quality spores.
HostingRepository	iProX
AnnounceDate	2024-06-07
AnnouncementXML	Submission_2024-07-15_21:08:37.959.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lei Rao
SpeciesList	scientific name: Bacillus subtilis PY79; NCBI TaxID: 1415167;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-10 19:33:59	ID requested
1	2024-07-10 21:22:14	announced
⏵ 2	2024-07-15 21:08:38	announced	2024-07-16: Update information.

Lyu F, Yang D, Rao L, Liao X, Alanine and glutamate catabolism collaborate to ensure the success of Bacillus subtilis sporulation. Microbiol Res, 286():127828(2024) [pubmed]

submitter keyword: TMT, Bacillus subtilis

Xiaojun Liao
contact affiliation	China Agricultural University
contact email	liaoxjun@cau.edu.cn
lab head
Lei Rao
contact affiliation	China Agricultural University
contact email	rao.lei@cau.edu.cn
dataset submitter

iProX dataset URI