PXD052966

PXD052966 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	GSK3 inhibition reduces ECM production and prevents age-related macular degeneration-like pathology
Description	All samples originated from ARPE-19 cultured cells. Cells were treated with either vehicle (DMSO, 1:1000) or CHIR99021 (1 uM) in serum free media for 7 days followed by harvesting the conditioned lysate, concentration using an Amicon filter, and loading onto an SDS-PAGE gel. Total protein (unsolved, in the stacking gel) was stained with Coomassie Blue and the gel bands containing total secreted protein were excised and submitted to the Proteomics Core (UT Southwestern).
HostingRepository	MassIVE
AnnounceDate	2024-06-10
AnnouncementXML	Submission_2024-06-10_10:19:41.958.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andrew Lemoff
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-10 10:07:23	ID requested
⏵ 1	2024-06-10 10:19:42	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: EFEMP1, fibulin-3, extracellular matrix, CHIR99021, Doyne Honeycomb Retinal Dystrophy, Malattia Leventinese, age-related macular degeneration, GSK3, ARPE-19

submitter keyword: EFEMP1, fibulin-3, extracellular matrix, CHIR99021, Doyne Honeycomb Retinal Dystrophy, Malattia Leventinese, age-related macular degeneration, GSK3, ARPE-19

Contact List

John D. Hulleman, Ph.D.
contact affiliation	University of Minnesota
contact email	hulleman@umn.edu
lab head
Andrew Lemoff
contact affiliation	UT-Southwestern Medical Center
contact email	andrew.lemoff@utsw.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v08/MSV000094967/

MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v08/MSV000094967/