PXD052964 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Structural basis for the assembly of Mis18a/b core modules |
Description | The centromere, defined by the enrichment of CENP-A (a Histone H3 variant) containing nucleosomes, is a specialised chromosomal locus that acts as a microtubule attachment site. To preserve centromere identity, CENP-A levels must be maintained through active CENP-A loading during the cell cycle. A central player mediating this process is the Mis18 complex (Mis18a, Mis18b and Mis18BP1), which recruits the CENP-A specific chaperone HJURP to centromeres for CENP-A deposition. Here, using a multi-pronged approach, we characterise the structure of the Mis18 complex and show that multiple hetero- and homo-oligomeric interfaces facilitate the hetero-octameric Mis18 complex assembly composed of 4 Mis18a, 2 Mis18b and 2 Mis18BP1. Evaluation of structure-guided/separation-of-function mutants reveals structural determinants essential for Mis18 complex assembly and centromere maintenance. Our results provide new mechanistic insights on centromere maintenance, highlighting that while Mis18a can associate with centromeres and deposit CENP-A independently of Mis18b, the latter is indispensable for the optimal level of CENP-A loading required for preserving the centromere identity. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:59:36.483.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Chandni Natalia Kumar |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | carbamoylated residue; phosphorylated residue |
Instrument | Bruker Daltonics timsTOF series |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-06-10 08:30:34 | ID requested | |
1 | 2024-09-25 03:39:48 | announced | |
⏵ 2 | 2024-10-22 06:59:36 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: centromere, mass spectrometry |
Contact List
Dr. Axel |
contact affiliation | Ludwig-Maximilians-Universität München Protein Analysis Unit Molecular Biology Department Großhadernerstr. 9 82152 Planegg-Martinsried |
contact email | imhof@lmu.de |
lab head | |
Chandni Natalia Kumar |
contact affiliation | Biomedical Center Munich, Ludwig-Maximilians Universität |
contact email | chandni.kumar@bmc.med.lmu.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/09/PXD052964 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD052964
- Label: PRIDE project
- Name: Structural basis for the assembly of Mis18a/b core modules