PXD052964

PXD052964 is an original dataset announced via ProteomeXchange.

Title	Structural basis for the assembly of Mis18a/b core modules
Description	The centromere, defined by the enrichment of CENP-A (a Histone H3 variant) containing nucleosomes, is a specialised chromosomal locus that acts as a microtubule attachment site. To preserve centromere identity, CENP-A levels must be maintained through active CENP-A loading during the cell cycle. A central player mediating this process is the Mis18 complex (Mis18a, Mis18b and Mis18BP1), which recruits the CENP-A specific chaperone HJURP to centromeres for CENP-A deposition. Here, using a multi-pronged approach, we characterise the structure of the Mis18 complex and show that multiple hetero- and homo-oligomeric interfaces facilitate the hetero-octameric Mis18 complex assembly composed of 4 Mis18a, 2 Mis18b and 2 Mis18BP1. Evaluation of structure-guided/separation-of-function mutants reveals structural determinants essential for Mis18 complex assembly and centromere maintenance. Our results provide new mechanistic insights on centromere maintenance, highlighting that while Mis18a can associate with centromeres and deposit CENP-A independently of Mis18b, the latter is indispensable for the optimal level of CENP-A loading required for preserving the centromere identity.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:59:36.483.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chandni Natalia Kumar
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	carbamoylated residue; phosphorylated residue
Instrument	Bruker Daltonics timsTOF series

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-10 08:30:34	ID requested
1	2024-09-25 03:39:48	announced
⏵ 2	2024-10-22 06:59:36	announced	2024-10-22: Updated project metadata.

10.1126/SCIENCE.ADO8270;

submitter keyword: centromere, mass spectrometry

Dr. Axel
contact affiliation	Ludwig-Maximilians-Universität München Protein Analysis Unit Molecular Biology Department Großhadernerstr. 9 82152 Planegg-Martinsried
contact email	imhof@lmu.de
lab head
Chandni Natalia Kumar
contact affiliation	Biomedical Center Munich, Ludwig-Maximilians Universität
contact email	chandni.kumar@bmc.med.lmu.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/09/PXD052964

PRIDE project URI

• PRIDE
  1. PXD052964
     1. Label: PRIDE project
     2. Name: Structural basis for the assembly of Mis18a/b core modules