PXD052958

PXD052958 is an original dataset announced via ProteomeXchange.

Title	Memantine administration enhances glutamatergic and GABAergic pathways in human hippocampus of Alzeimer´s disease patients
Description	Alzheimer´s disease (AD) represents a serious health, social and economic problem. One of the traditional treatment is administration of memantine, the NMDA receptor antagonist. In this study we investigated how memantine medication impacts protein expression in hippocampus of AD patients using TMT-based quantitative proteomics applied on formalin-fixed paraffin embedded post mortem tissues. The presented dataset shows that memantine treatment has minor but distinct effect on protein expression. While memantine did not prevented general decline of mitochondrial proteome seen in unmedicated AD patients, the treatment selectively induced levels of several mitochondrially-encoded proteins. Furthermore, memantine medication mitigated proteomic pattern of activated phagocytes and enhanced expression of synaptic components involved in both inhibitory (GABA) and excitatory (glutamate) neurotransmission. The upregulation was specific for proteins for which glutamate/GABA serves as a ligand or a substrate (i.e. receptors, transporters, enzymes, etc.), without affecting neuronal nor post-synaptic density markers. This may indicate that memantine-associated effects on glutamatergic and GABAergic is a reaction to memantine-mediated changes of glutamate and GABA levels.
HostingRepository	PRIDE
AnnounceDate	2026-02-09
AnnouncementXML	Submission_2026-02-08_20:49:38.671.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ivo Fabrik
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-10 04:17:56	ID requested
⏵ 1	2026-02-08 20:49:42	announced

Fabrik I, Kupcik R, Fabrikova D, Chvojkova M, Holubova K, Hakenova K, Horak M, Soukup J, Manethova M, Rusina R, Matej R, Ryska A, Soukup O, Memantine Administration Enhances Glutamatergic and GABAergic Pathways in the Human Hippocampus of Alzheimer's Disease Patients. Proteomics, 25(15):42-49(2025) [pubmed]

10.1002/pmic.70006;

submitter keyword: memantine

FFPE

Alzheimer´s disease

proteomics

hippocampus

glutamate receptors

metabolism

Ondrej Soukup
contact affiliation	Biomedical Research Centre, University Hospital Hradec Kralove, Sokolska 581, 500 05 Hradec Kralove, Czech Republic
contact email	ondrej.soukup@fnhk.cz
lab head
Ivo Fabrik
contact affiliation	Biomedical Research Center, University Hospital Hradec Kralove
contact email	ivo.fabrik@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD052958

PRIDE project URI

• PRIDE
  1. PXD052958
     1. Label: PRIDE project
     2. Name: Memantine administration enhances glutamatergic and GABAergic pathways in human hippocampus of Alzeimer´s disease patients