PXD052946

PXD052946 is an original dataset announced via ProteomeXchange.

Title	Traffic jam directly regulates the Drosophila piRNA cluster flamenco and the Piwi pathway gene network to ensure transposon silencing and female fertility.
Description	The flamenco (flam) locus is the most prominent piRNA cluster locus expressed in Drosophila ovarian follicle cells that is required for female fertility and silencing of gypsy/mdg4 transposons. Although P-element insertions in the flam promoter region disrupts flam piRNAs and cause female infertility, the flam enhancer architecture is still poorly understood. To illuminate flam’s regulation, we uncovered novel shadow enhancer (SE) sequences within the first exons of flam via promoter-bashing assays in OSS cells. We confirmed the SE relevance in vivo with new Drosophila flam deletion mutants of these regions that compromised flam piRNA expression and lowered female fertility from unleashed transposon expression. In a proteomic analysis of proteins associated with these SE sequences, the MAF transcription factor Traffic jam (Tj) emerged as the most prominent binding candidate. With Tj knockdowns in OSS cells, we see a coordinated loss of flam transcripts and piRNAs as well as a decreased expression of many Piwi pathway genes including piwi, which then also coincided with increased transposon RNA levels. We conducted ChIP-seq of Tj’s interaction at Piwi pathway gene promoters and hundreds of other genes important for follicle cell development. Lastly, we utilized transient Tj knockdown in the fly ovary to support our conclusions of how Tj orchestrates the complex Piwi pathway network in Drosophila follicle cells.
HostingRepository	PRIDE
AnnounceDate	2025-04-01
AnnouncementXML	Submission_2025-04-01_10:11:11.967.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD052946
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Raghuveera Goel
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-09 04:14:16	ID requested
⏵ 1	2025-04-01 10:11:12	announced

10.6019/PXD052946;

Rivera A, Lee JR, Gupta S, Yang L, Goel RK, Zaia J, Lau NC, fertility. bioRxiv, ():(2024) [pubmed]

10.1101/2024.08.15.608167;

submitter keyword: transcription, TMT, multiplexed proteomics, Traffic jam, Affinity enrichment, transposon, piRNA, fertilitym transposon,Drosophila melanogaster

Nelson Lau
contact affiliation	Department of Biochemistry and Cell Biology, Boston University Chobanian and Avedisian School of Medicine
contact email	nclau@bu.edu
lab head
Raghuveera Goel
contact affiliation	Boston University
contact email	rgoel@bu.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD052946
     1. Label: PRIDE project
     2. Name: Traffic jam directly regulates the Drosophila piRNA cluster flamenco and the Piwi pathway gene network to ensure transposon silencing and female fertility.