PXD052935

PXD052935 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Cysteine capping with CAM reagent
Description	CAM reagent was synthesized and used to uniquely convert cysteine residues, under mild conditions, into trypsin-recognsable sites. We apply this cysteine-capping methodology in the identification of missense variants in HCT-15 cell lines.2-bromoethylamine (BEA)was used as controls, for cysteine capping and subjected to similar trypsin treatment.
HostingRepository	PRIDE
AnnounceDate	2025-03-13
AnnouncementXML	Submission_2025-03-13_06:06:26.079.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Samuel Ofori
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-07 12:10:53	ID requested
⏵ 1	2025-03-13 06:06:26	announced

Publication List

10.1039/d4cc01583e;
Ofori S, Desai HS, Shikwana F, Boatner LM, Dominguez Iii ER, Castell, ó, n JO, Backus KM, Generating cysteine-trypsin cleavage sites with 2-chloroacetamidine capping. Chem Commun (Camb), 60(67):8856-8859(2024) [pubmed]

10.1039/d4cc01583e;

Ofori S, Desai HS, Shikwana F, Boatner LM, Dominguez Iii ER, Castell, ó, n JO, Backus KM, Generating cysteine-trypsin cleavage sites with 2-chloroacetamidine capping. Chem Commun (Camb), 60(67):8856-8859(2024) [pubmed]

Keyword List

submitter keyword: CAM,cysteine capping, aminoethylation

submitter keyword: CAM,cysteine capping, aminoethylation

Contact List

Dr Keriann Marie Backus
contact affiliation	Biological Chemistry, University of California Los Angeles, United States
contact email	kbackus@mednet.ucla.edu
lab head
Samuel Ofori
contact affiliation	UCLA
contact email	sofori@mednet.ucla.edu
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/03/PXD052935
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/03/PXD052935

PRIDE project URI

Repository Record List

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