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PXD052935

PXD052935 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCysteine capping with CAM reagent
DescriptionCAM reagent was synthesized and used to uniquely convert cysteine residues, under mild conditions, into trypsin-recognsable sites. We apply this cysteine-capping methodology in the identification of missense variants in HCT-15 cell lines.2-bromoethylamine (BEA)was used as controls, for cysteine capping and subjected to similar trypsin treatment.
HostingRepositoryPRIDE
AnnounceDate2025-03-13
AnnouncementXMLSubmission_2025-03-13_06:06:26.079.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSamuel Ofori
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Eclipse
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-06-07 12:10:53ID requested
12025-03-13 06:06:26announced
Publication List
10.1039/d4cc01583e;
Ofori S, Desai HS, Shikwana F, Boatner LM, Dominguez Iii ER, Castell, รณ, n JO, Backus KM, Generating cysteine-trypsin cleavage sites with 2-chloroacetamidine capping. Chem Commun (Camb), 60(67):8856-8859(2024) [pubmed]
Keyword List
submitter keyword: CAM,cysteine capping, aminoethylation
Contact List
Dr Keriann Marie Backus
contact affiliationBiological Chemistry, University of California Los Angeles, United States
contact emailkbackus@mednet.ucla.edu
lab head
Samuel Ofori
contact affiliationUCLA
contact emailsofori@mednet.ucla.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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