PXD052887

PXD052887 is an original dataset announced via ProteomeXchange.

Title	Proteomic insights into composition-dependent effects of microplastics on freshwater microalgae Chlamydomonas reinhardtii
Description	The widespread presence of microplastics (MPs) in freshwater systems has raised concerns about their potential ecotoxicity on aquatic organisms. In this study, we evaluated the effects of four MPs with different compositions, namely polyethylene (PE), polystyrene (PS), polyvinyl chloride (PVC) and polypropylene (PP), on freshwater microalgae Chlamydomonas reinhardtii. PS and PVC MPs caused greater growth inhibition and stronger oxidative stress response than PP and PE MPs. Proteomics analysis was employed to explore the mechanical understanding of the composition-dependent toxicity of MPs on microalgae. Proteins involved in photosynthesis processes were identified as contributors to the diverse responses of microalgae to differently composed MPs. Photosynthesis activity of algae, including pigment content and photoprotective response, was determined to reflect the distinct effect of the four MPs. The indicated down-regulated expression of photosynthetic proteins by proteomics analysis was further confirmed using western blot, with PVC and PS showing greater impacts on their expression reduction. Our findings not only show the composition-dependent effect of MPs on microalgae but also provide important insights into the molecular mechanism of MPs’ toxicity on natural phytoplankton species.
HostingRepository	iProX
AnnounceDate	2024-06-06
AnnouncementXML	Submission_2024-06-05_22:35:24.463.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chu Zheng
SpeciesList	scientific name: Chlamydomonas reinhardtii; NCBI TaxID: 3055;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-05 22:34:29	ID requested
⏵ 1	2024-06-05 22:35:24	announced

Dataset with its publication pending

submitter keyword: Microplastics, Growth inhibition, Oxidative stress, Proteomics, Photosynthesis process

Jigang Wang
contact affiliation	State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, Artemisinin Research Center, and Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences
contact email	jgwang@icmm.ac.cn
lab head
Chu Zheng
contact affiliation	State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, Artemisinin Research Center, and Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences
contact email	cuzer_1997@163.com
dataset submitter

iProX dataset URI