PXD052860

PXD052860 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Effects of gradient lengths and acquisition frequency on the number of protein identifications in nanoflow LCMSMS of complex proteomic sample homo sapiens
Description	A liquid chromatography-tandem mass-spectrometry LC-MS/MS is now an indispensable method for shotgun proteomics. The combination of chromatographic separation and MS/MS identification provides valuable information about protein compositions from different biological samples. In this article, we examined the relationship between gradient length and MS acquisition on examples of three complex peptide mixtures. We found that combining long gradient and higher MS acquisitions resulted in more protein identifications for human plasma, Escherichia coli, and Chinese hamster ovary cell lines. We also evaluated the efficiency of Mascot and PEAKS proteomic tools for the analysis of LC-MS/MS data.
HostingRepository	MassIVE
AnnounceDate	2024-07-02
AnnouncementXML	Submission_2024-07-02_02:13:26.963.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Arman
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	Oxidation; Carbamidomethyl; Acetyl
Instrument	impact II

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-05 06:00:12	ID requested
⏵ 1	2024-07-02 02:13:27	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: Complex proteomics samples, data-dependent acquisition (DDA), liquid chromatography-tandem mass-spectrometry LC-MS/MS, in-solution digestion

submitter keyword: Complex proteomics samples, data-dependent acquisition (DDA), liquid chromatography-tandem mass-spectrometry LC-MS/MS, in-solution digestion

Contact List

Arman Kulyyassov
contact affiliation	National Center for Biotechnology Ministry of Healthcare of the Republic of Kazakhstan
contact email	kulyyasov@biocenter.kz
lab head
Arman
contact affiliation	National center for biotechnology
contact email	kulyyasov@biocenter.kz
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v08/MSV000094936/