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PXD052848

PXD052848 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAlternatively spliced mini exon B in PTPRD regulates hippocampal excitatory synapses through trans synaptic PTPRD IL1RAP interaction
DescriptionPTPRD is a presynaptic adhesion molecule implicated in various neurological, psychiatric, and neurodevelopmental disorders, but the underlying mechanisms remain unclear. PTPRD trans synaptically interacts with multiple postsynaptic adhesion molecules, thorough the regulation by its extracellular alternatively spliced mini exons (meA and meB). While PTPRD meA regulates specific hippocampal excitatory synapses, PTPRD meB has not been studied in vivo. Here we found that homozygous PTPRD meB mutant (Ptprd meB homo del) mice show early postnatal lethality and that heterozygous Ptprd meB hetero del mice show behavioral and synaptic abnormalities, which are not observed in PTPRD meA mutant mice. In Ptprd-meB hetero del mice, excitatory synapses in dentate gyrus granule cells (DG GCs) show decreased density and transmission, whereas PTPRD meA mutant mice show normal excitatory synaptic transmission. Proteomic analyses indicate decreased postsynaptic levels of IL1RAP, a known trans synaptic partner of PTPRD meB. Accordingly, IL1RAP mutant mice show similarly decreased excitatory synaptic transmission in DG GCs. Therefore, PTPRD meB is more important than PTPRD meA for postnatal survival, excitatory synaptic function, and mouse behaviors, and IL1RAP mediates PTPRD meB dependent excitatory synaptic regulation.
HostingRepositoryMassIVE
AnnounceDate2025-03-31
AnnouncementXMLSubmission_2025-03-31_18:43:18.697.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterYeji Yang
SpeciesList scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationListCarbamidomethyl; Oxidation
InstrumentOrbitrap Eclipse
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-06-04 22:21:22ID requested
12025-03-31 18:43:19announced
Publication List
no publication
Keyword List
submitter keyword: Proteomics, PTPRD, hippocampal excitatory synapses, presynaptic adhesion molecule, neurodevelopmental disorders
Contact List
Jin Young Kim
contact affiliationKorea Basic Science Institute
contact emailjinyoung@kbsi.re.kr
lab head
Yeji Yang
contact affiliationKorea Basic Science Institute
contact emailyangyj@kbsi.re.kr
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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