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PXD052826

PXD052826 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCalcium-triggered (de)ubiquitination events in depolarized synaptosomes
DescriptionNeurons communicate at synapses through the release of neurotransmitters (NT). These NTs are stored in synaptic vesicles (SVs) at the presynaptic nerve terminal, where they undergo a trafficking cycle consisting of Ca2+-triggered SV exocytosis, SV endocytosis and regeneration. While protein phosphorylation is known to fine-tune the SV trafficking cycle, another post-translational modification, protein ubiquitination, has been recently linked to NT release. However, the proteins and sites that undergo (de)ubiquitination at the synapse in response to Ca2+ influx remain unknown. Using a mass-spectrometry-based, quantification analysis of ubiquitinated proteins in isolated rat synaptosomes, we identified more than 5,000 ubiquitination sites on approximately 2,000 proteins. Among these, 41 proteins showed significant ubiquitination changes in response to Ca2+ influx, with Ca2+/calmodulin-dependent kinase II α (CaMKIIα) and the clathrin adaptor protein, AP180, showing the most pronounced changes. By expressing a fluorescence resonance energy transfer (FRET) probe, Camui, along with a mutant form lacking the ubiquitination target lysine site (K291), in non-neuronal and neuronal cells, we showed that K291 ubiquitination partially attenuates CaMKIIα activity and synaptic function.
HostingRepositoryPRIDE
AnnounceDate2025-04-18
AnnouncementXMLSubmission_2025-04-18_05:50:15.340.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSofia Ainatzi
SpeciesList scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; ubiquitination signature dipeptidyl lysine; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Exploris 480; Q Exactive HF-X
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-06-04 07:13:13ID requested
12025-04-18 05:50:15announced
Publication List
10.1016/J.MCPRO.2025.100946;
Keyword List
submitter keyword: LC-MSMS, synaptosomes,rat, TMT, PRM, CaMKIIa, ubiquitin, DDA
Contact List
Henning Urlaub
contact affiliationMax Planck Institute for Multidisciplinary Sciences
contact emailhenning.urlaub@mpinat.mpg.de
lab head
Sofia Ainatzi
contact affiliationMax Planck Institute for Multidisciplinary Sciences
contact emailsofia.ainatzi@mpinat.mpg.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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