PXD052826

PXD052826 is an original dataset announced via ProteomeXchange.

Title	Calcium-triggered (de)ubiquitination events in depolarized synaptosomes
Description	Neurons communicate at synapses through the release of neurotransmitters (NT). These NTs are stored in synaptic vesicles (SVs) at the presynaptic nerve terminal, where they undergo a trafficking cycle consisting of Ca2+-triggered SV exocytosis, SV endocytosis and regeneration. While protein phosphorylation is known to fine-tune the SV trafficking cycle, another post-translational modification, protein ubiquitination, has been recently linked to NT release. However, the proteins and sites that undergo (de)ubiquitination at the synapse in response to Ca2+ influx remain unknown. Using a mass-spectrometry-based, quantification analysis of ubiquitinated proteins in isolated rat synaptosomes, we identified more than 5,000 ubiquitination sites on approximately 2,000 proteins. Among these, 41 proteins showed significant ubiquitination changes in response to Ca2+ influx, with Ca2+/calmodulin-dependent kinase II α (CaMKIIα) and the clathrin adaptor protein, AP180, showing the most pronounced changes. By expressing a fluorescence resonance energy transfer (FRET) probe, Camui, along with a mutant form lacking the ubiquitination target lysine site (K291), in non-neuronal and neuronal cells, we showed that K291 ubiquitination partially attenuates CaMKIIα activity and synaptic function.
HostingRepository	PRIDE
AnnounceDate	2025-04-18
AnnouncementXML	Submission_2025-04-18_05:50:15.340.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sofia Ainatzi
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; ubiquitination signature dipeptidyl lysine; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480; Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-04 07:13:13	ID requested
⏵ 1	2025-04-18 05:50:15	announced

10.1016/J.MCPRO.2025.100946;

submitter keyword: LC-MSMS, synaptosomes,rat, TMT, PRM, CaMKIIa, ubiquitin, DDA

Henning Urlaub
contact affiliation	Max Planck Institute for Multidisciplinary Sciences
contact email	henning.urlaub@mpinat.mpg.de
lab head
Sofia Ainatzi
contact affiliation	Max Planck Institute for Multidisciplinary Sciences
contact email	sofia.ainatzi@mpinat.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD052826
     1. Label: PRIDE project
     2. Name: Calcium-triggered (de)ubiquitination events in depolarized synaptosomes