PXD052826 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Calcium-triggered (de)ubiquitination events in depolarized synaptosomes |
Description | Neurons communicate at synapses through the release of neurotransmitters (NT). These NTs are stored in synaptic vesicles (SVs) at the presynaptic nerve terminal, where they undergo a trafficking cycle consisting of Ca2+-triggered SV exocytosis, SV endocytosis and regeneration. While protein phosphorylation is known to fine-tune the SV trafficking cycle, another post-translational modification, protein ubiquitination, has been recently linked to NT release. However, the proteins and sites that undergo (de)ubiquitination at the synapse in response to Ca2+ influx remain unknown. Using a mass-spectrometry-based, quantification analysis of ubiquitinated proteins in isolated rat synaptosomes, we identified more than 5,000 ubiquitination sites on approximately 2,000 proteins. Among these, 41 proteins showed significant ubiquitination changes in response to Ca2+ influx, with Ca2+/calmodulin-dependent kinase II α (CaMKIIα) and the clathrin adaptor protein, AP180, showing the most pronounced changes. By expressing a fluorescence resonance energy transfer (FRET) probe, Camui, along with a mutant form lacking the ubiquitination target lysine site (K291), in non-neuronal and neuronal cells, we showed that K291 ubiquitination partially attenuates CaMKIIα activity and synaptic function. |
HostingRepository | PRIDE |
AnnounceDate | 2025-04-18 |
AnnouncementXML | Submission_2025-04-18_05:50:15.340.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Sofia Ainatzi |
SpeciesList | scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; ubiquitination signature dipeptidyl lysine; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Exploris 480; Q Exactive HF-X |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-06-04 07:13:13 | ID requested | |
⏵ 1 | 2025-04-18 05:50:15 | announced | |
Publication List
Keyword List
submitter keyword: LC-MSMS, synaptosomes,rat, TMT, PRM, CaMKIIa, ubiquitin, DDA |
Contact List
Henning Urlaub |
contact affiliation | Max Planck Institute for Multidisciplinary Sciences |
contact email | henning.urlaub@mpinat.mpg.de |
lab head | |
Sofia Ainatzi |
contact affiliation | Max Planck Institute for Multidisciplinary Sciences |
contact email | sofia.ainatzi@mpinat.mpg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD052826
- Label: PRIDE project
- Name: Calcium-triggered (de)ubiquitination events in depolarized synaptosomes