PXD052824

PXD052824 is an original dataset announced via ProteomeXchange.

Title	A snapshot of the Physcomitrella N-terminome reveals N-terminal methylation of organellar proteins
Description	Post- or co-translational modifications of protein N-termini influence their half-life and mediate protein sorting to organelles via cleavable N-terminal sequences that are recognized by the respective translocation machinery. In the present study, we provide an overview on the current modification state of the N-termini of over 4500 proteins from the model moss species Physcomitrella (Physcomitrium patens) using a compilation of 24 N-terminomics datasets. Our data reveal distinct proteoforms and modification states and confirm predicted targeting peptide cleavage sites of 1144 proteins localized to plastids and the thylakoid lumen, to mitochondria, and to the secretory pathway, respectively. Additionally, we uncover extended N-terminal methylation of mitochondrial proteins. Moreover, we identified PpNTM1 (P. patens alpha N-terminal protein methyltransferase 1) as a promising candidate for protein methylation in plastids, mitochondria and the cytosol. These data can now be used to optimize computational targeting predictors, for customized protein fusions and their targeted localization in biotechnology, and it provides novel insights into potential dual targeting of proteins.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-06_18:28:57.187.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD052824
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ralf Reski
SpeciesList	scientific name: Physcomitrella patens subsp. patens (Moss); NCBI TaxID: 3218;
ModificationList	monomethylated residue; acetylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-04 06:59:38	ID requested
⏵ 1	2025-05-06 18:28:58	announced

10.6019/PXD052824;

Hoernstein SNW, Schlosser A, Fiedler K, van Gessel N, Igloi GL, Lang D, Reski R, A snapshot of the Physcomitrella N-terminome reveals N-terminal methylation of organellar proteins. Plant Cell Rep, 43(10):250(2024) [pubmed]

10.1007/s00299-024-03329-1;

submitter keyword: Physcomitrium, methylation, moss, mass spectrometry, N-terminus

Ralf Reski
contact affiliation	Head, Chair Plant Biotechnology Faculty of Biology University of Freiburg Schaenzlestrasse 1 D-79104 Freiburg Germany
contact email	ralf.reski@biologie.uni-freiburg.de
lab head
Ralf Reski
contact affiliation	Faculty of Biology, University of Freiburg (Chair Plant Biotechnology), Schaenzlestr. 1, D-79104 Freiburg
contact email	ralf.reski@biologie.uni-freiburg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD052824

PRIDE project URI

• PRIDE
  1. PXD052824
     1. Label: PRIDE project
     2. Name: A snapshot of the Physcomitrella N-terminome reveals N-terminal methylation of organellar proteins