PXD052817

PXD052817 is an original dataset announced via ProteomeXchange.

Title	Linear Protein Identification of Soluble and Insoluble Fractions from In-Situ DSSO Crosslinked Human Mitochondria
Description	In this project, freshly isolated mitochondria from K-562 cells were crosslinked immediately with the homobifunctional and membrane-permeable reagent disuccinimidyl sulfoxide (DSSO). The study focuses on the comprehensive identification of proteins, specifically distinguishing between soluble and insoluble fractions. Both fractions were digested using Trypsin and analyzed using Liquid Chromatography coupled with Tandem Mass Spectrometry (LC-MS/MS) and Data-Dependent Acquisition (DDA) methods. The resulting data were processed using MaxQuant software.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_01:54:05.433.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD052817
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Fabian Schildhauer
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-04 01:27:22	ID requested
⏵ 1	2025-05-06 01:54:05	announced

10.6019/PXD052817;

submitter keyword: DDA, LC-MSMS, insoluble, soluble, DSSO, Lumos, mitochondria, iBAQ, crosslinking

Juri Rappsilber
contact affiliation	Technische Universität Berlin, Chair of Bioanalytics, 10623 Berlin, Germany
contact email	juri.rappsilber@tu-berlin.de
lab head
Fabian Schildhauer
contact affiliation	Technische Universität Berlin
contact email	schildhauer@tu-berlin.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD052817
     1. Label: PRIDE project
     2. Name: Linear Protein Identification of Soluble and Insoluble Fractions from In-Situ DSSO Crosslinked Human Mitochondria