PXD052809 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | In Vitro Investigation of The Anti-Fibrotic Potential of 1-Phenyl-2-Pentanol, Identified from Moringa Oleifera Lam. on Hepatic Stellate Cells |
Description | Liver fibrosis, characterized by excessive extracellular matrix deposition, is driven by activated hepatic stellate cells (HSCs). Due to the limited availability of anti-fibrotic drugs, research con-tinues to explore potential therapeutic agents. Moringa oleifera Lam. (MO), known for its various bioactive properties, is being investigated for its anti-fibrotic potential. This study focused on 1-phenyl-2-pentanol (1-PHE), a compound derived from MO leaves, and its impact on LX-2 hu-man hepatic stellate cell activation. TGF-β1-stimulated LX-2 cells were treated with MO extract or 1-PHE, and liver fibrosis markers were assessed at both gene and protein levels. Proteomic analysis and molecular docking were employed to identify potential protein targets and signaling pathways affected by 1-PHE. 1-PHE treatment downregulated fibrosis markers, including colla-gen type I alpha 1 chain (COL1A1), collagen type IV alpha 1 chain (COL4A1), mothers against decapentaplegic homolog 2 and 3 (SMAD2/3), and matrix metalloproteinase-2 (MMP2), and re-duced the secretion of matrix metalloproteinase-9 (MMP9). Proteomic analysis revealed the pos-sible mechanism of 1-PHE in modulating the Wnt/β-catenin pathway. These findings suggest that 1-PHE may suppress HSCs activation by inhibiting the TGF-β1 and Wnt/β-catenin signaling pathways, indicating its potential as an anti-liver fibrosis agent. Further research is warranted to validate these findings. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-17 |
AnnouncementXML | Submission_2024-10-17_05:11:49.460.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Watunyoo Buakaew |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized amino-terminal residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-06-03 19:53:05 | ID requested | |
⏵ 1 | 2024-10-17 05:11:50 | announced | |
Publication List
10.3390/ijms25168995; |
Buakaew W, Krobthong S, Yingchutrakul Y, Khamto N, Sutana P, Potup P, Thongsri Y, Daowtak K, Ferrante A, L, é, on C, Usuwanthim K, Lam., on Hepatic Stellate Cells. Int J Mol Sci, 25(16):(2024) [pubmed] |
Keyword List
submitter keyword: Hepatic stellate cell, Liver fibrosis, Moringa oleifera Lam., Molecular docking analysis, Proteomics,1-phenyl-2-pentanol, LC-MS/MS |
Contact List
Kanchana Usuwanthim |
contact affiliation | Cellular and Molecular Immunology Research Unit (CMIRU), Faculty of Allied Health Sciences, Naresuan University, Phitsanulok, 65000, Thailand |
contact email | kanchanau@nu.ac.th |
lab head | |
Watunyoo Buakaew |
contact affiliation | Department of Microbiology, Faculty of Medicine, Srinakharinwirot University |
contact email | watunyoo@g.swu.ac.th |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD052809
- Label: PRIDE project
- Name: In Vitro Investigation of The Anti-Fibrotic Potential of 1-Phenyl-2-Pentanol, Identified from Moringa Oleifera Lam. on Hepatic Stellate Cells