PXD052809

PXD052809 is an original dataset announced via ProteomeXchange.

Title	In Vitro Investigation of The Anti-Fibrotic Potential of 1-Phenyl-2-Pentanol, Identified from Moringa Oleifera Lam. on Hepatic Stellate Cells
Description	Liver fibrosis, characterized by excessive extracellular matrix deposition, is driven by activated hepatic stellate cells (HSCs). Due to the limited availability of anti-fibrotic drugs, research con-tinues to explore potential therapeutic agents. Moringa oleifera Lam. (MO), known for its various bioactive properties, is being investigated for its anti-fibrotic potential. This study focused on 1-phenyl-2-pentanol (1-PHE), a compound derived from MO leaves, and its impact on LX-2 hu-man hepatic stellate cell activation. TGF-β1-stimulated LX-2 cells were treated with MO extract or 1-PHE, and liver fibrosis markers were assessed at both gene and protein levels. Proteomic analysis and molecular docking were employed to identify potential protein targets and signaling pathways affected by 1-PHE. 1-PHE treatment downregulated fibrosis markers, including colla-gen type I alpha 1 chain (COL1A1), collagen type IV alpha 1 chain (COL4A1), mothers against decapentaplegic homolog 2 and 3 (SMAD2/3), and matrix metalloproteinase-2 (MMP2), and re-duced the secretion of matrix metalloproteinase-9 (MMP9). Proteomic analysis revealed the pos-sible mechanism of 1-PHE in modulating the Wnt/β-catenin pathway. These findings suggest that 1-PHE may suppress HSCs activation by inhibiting the TGF-β1 and Wnt/β-catenin signaling pathways, indicating its potential as an anti-liver fibrosis agent. Further research is warranted to validate these findings.
HostingRepository	PRIDE
AnnounceDate	2024-10-17
AnnouncementXML	Submission_2024-10-17_05:11:49.460.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Watunyoo Buakaew
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized amino-terminal residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-03 19:53:05	ID requested
⏵ 1	2024-10-17 05:11:50	announced

10.3390/ijms25168995;

Buakaew W, Krobthong S, Yingchutrakul Y, Khamto N, Sutana P, Potup P, Thongsri Y, Daowtak K, Ferrante A, L, é, on C, Usuwanthim K, Lam., on Hepatic Stellate Cells. Int J Mol Sci, 25(16):(2024) [pubmed]

submitter keyword: Hepatic stellate cell, Liver fibrosis, Moringa oleifera Lam., Molecular docking analysis, Proteomics,1-phenyl-2-pentanol, LC-MS/MS

Kanchana Usuwanthim
contact affiliation	Cellular and Molecular Immunology Research Unit (CMIRU), Faculty of Allied Health Sciences, Naresuan University, Phitsanulok, 65000, Thailand
contact email	kanchanau@nu.ac.th
lab head
Watunyoo Buakaew
contact affiliation	Department of Microbiology, Faculty of Medicine, Srinakharinwirot University
contact email	watunyoo@g.swu.ac.th
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD052809
     1. Label: PRIDE project
     2. Name: In Vitro Investigation of The Anti-Fibrotic Potential of 1-Phenyl-2-Pentanol, Identified from Moringa Oleifera Lam. on Hepatic Stellate Cells