PXD052802

PXD052802 is an original dataset announced via ProteomeXchange.

Title	Vault Interactome in WT and PARP4-Depleted A549 Cells
Description	We sought to determine whether the vault complex component Poly (ADP-ribose) Polymerase 4 (PARP4) would impact the identity or relative enrichment of proteins that interacted with the vault. We conducted co-immunoprecipitation (co-IP) experiments targeting the human major vault protein (MVP) using a synthetic antigen binding reagent (sAB) generated for this study. Experimental co-IP's were conducted by adding the anti-MVP sAB (called VM1) to WT A549 cells and to cells from which PARP4 had been depleted using CRISPR/Cas9. To control for background, we concurrently processed samples to which we had added an anti-GFP sAB (called H6) that was highly similar to our anti-MVP reagent but was not specific for any human proteins. PARP4, as well as a number of other proteins, were significantly enriched in the experimental co-IP from the WT versus PARP4 KO cells. Overall, we identified 34 hits that were either unique to or differentially enriched in the WT cell experimental co-IP, 16 hits that were unique to the PARP4 KO experimental co-IP, and 18 "shared" hits that were present in each experimental co-IP dataset without significant differential enrichment in either.
HostingRepository	PRIDE
AnnounceDate	2025-07-14
AnnouncementXML	Submission_2025-07-13_17:43:38.918.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jane Lodwick
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-03 13:28:40	ID requested
⏵ 1	2025-07-13 17:43:39	announced

Dataset with its publication pending

submitter keyword: lung non-small cell carcinoma, PARP4, Human, MVP, A549, alveolar basal epithelial cells,Vault

Minglei Zhao
contact affiliation	United States, Chicago, University of Chicago, Department of Biochemistry & Molecular Biophysics
contact email	mlzhao@uchicago.edu
lab head
Jane Lodwick
contact affiliation	University of Chicago
contact email	jlodwick3@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD052802

PRIDE project URI

• PRIDE
  1. PXD052802
     1. Label: PRIDE project
     2. Name: Vault Interactome in WT and PARP4-Depleted A549 Cells