PXD052790

PXD052790 is an original dataset announced via ProteomeXchange.

Title	Tracking DOT1L Methyltransferase Activity by Stable Isotope Labelling Using a Selective Synthetic Co-Factor
Description	Histone methylation is a post-translational modification (PTM) regulating gene expression. We developed an isotopically labelled analogue of co-factor S-adenosyl-L-methionine (13CD3-BrSAM), with selectivity for the histone lysine methyltransferase DOT1L, permitting tracking of methylation activity by mass spectrometry (MS). This concept could be applied to other methyltransferases, linking PTM discovery to enzymatic mediators.
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_13:53:49.683.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Harry Whitwell
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monomethylated residue; monohydroxylated residue; symmetric dimethyl-L-arginine; iodoacetamide derivatized residue; N6,N6-dimethyl-L-lysine
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-03 03:09:43	ID requested
⏵ 1	2024-10-07 13:53:50	announced

10.1038/S42004-024-01227-X;

submitter keyword: DOT1L methylation epigenetics histones

Harry Whitwell
contact affiliation	Division of Systems Medicine, Imperial College London
contact email	h.whitwell@imperial.ac.uk
lab head
Harry Whitwell
contact affiliation	Imperial College London
contact email	h.whitwell@imperial.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/06/PXD052790

PRIDE project URI

• PRIDE
  1. PXD052790
     1. Label: PRIDE project
     2. Name: Tracking DOT1L Methyltransferase Activity by Stable Isotope Labelling Using a Selective Synthetic Co-Factor