PXD052748

PXD052748 is an original dataset announced via ProteomeXchange.

Title	Glycosylation landscape of CAR-T in AAVS1 and SPPL3 knockout
Description	In this study, we delved into deciphering the glycosylation patterns and precise localization of glycosylation sites within CAR-T proteins. Specifically, we focused on samples derived from human T cells that underwent gene knockout of AAVS1 and SPPL3, two critical genes that could potentially influence CAR-T function. To ensure accuracy and precision, we employed optimal tribrid orbitrap mass spectrometry settings for glycosylation landscapes of the CAR-T proteins. By analyzing these data, we aimed to gain a deeper understanding of how AAVS1 and SPPL3 knockout impacted the glycosylation profiles and ultimately the function and efficacy of CAR-T cells in immunotherapy applications.
HostingRepository	PRIDE
AnnounceDate	2025-05-23
AnnouncementXML	Submission_2025-05-23_09:00:11.639.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xue Sun
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	complex glycosylation
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-31 02:54:30	ID requested
⏵ 1	2025-05-23 09:00:12	announced

Dataset with its publication pending

submitter keyword: CAR-T, SPPL3, AAVS1,Glycosylation, LC-MS/MS

Catherine CL Wong
contact affiliation	State Key Laboratory of Complex, Severe and Rare Diseases, Clinical Research Institute, Peking Union Medical College Hospital, Chinese Academy of Medical Science & Peking Union Medical College, Beijing, China
contact email	catclw321@126.com
lab head
Xue Sun
contact affiliation	Peking University
contact email	sun.x@stu.pku.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD052748

PRIDE project URI

• PRIDE
  1. PXD052748
     1. Label: PRIDE project
     2. Name: Glycosylation landscape of CAR-T in AAVS1 and SPPL3 knockout