PXD052747

PXD052747 is an original dataset announced via ProteomeXchange.

Title	Aspartate signaling increases the aggressiveness of lung metastases by inducing eIF5A-mediated translation
Description	Lung metastases are detected in more than half of patients with metastatic tumors, yet it remains largely unknown why the lung environment is particularly permissive to metastases. Here, we describe that pulmonary aspartate promotes lung metastasis by rewiring the translatome of disseminating cancer cells. We found that both patients and mice with breast cancer have high concentrations of aspartate in their lung interstitial fluid. This extracellular aspartate activates the ionotropic N-methyl-D-aspartate (NMDA) receptor in cancer cells, which induces CREB-dependent mRNA expression of deoxyhypusine hydroxylase (DOHH). The latter is essential for hypusination, a posttranslational modification required for the activity of the non-classical translation initiation factor eIF5A. In turn, a translational program with TGF- signaling as a central hub promotes collagen remodeling in the disseminated breast cancer cells. We detect key aspects of this mechanism in lung metastases from patients with breast cancer. In summary, we discover that pulmonary aspartate increases with breast cancer and induces a signaling cascade that promote lung metastatic outgrowth.
HostingRepository	PRIDE
AnnounceDate	2026-09-01
AnnouncementXML	Submission_2026-09-01_03:55:52.889.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yu-Heng Hsieh
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	hypusine
Instrument	Orbitrap Exploris 480; Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-31 02:05:55	ID requested
⏵ 1	2026-09-01 03:55:53	announced

10.1038/S41586-024-08335-7;

submitter keyword: PTM,mass spectrometry, hypusine

Prof. Dr. Albert Sickmann
contact affiliation	Leibniz Institut für Analytische Wissenschaften-ISAS-e.V., Dortmund, Germany
contact email	sickmann@isas.de
lab head
Yu-Heng Hsieh
contact affiliation	Leibniz-Institut für Analytische Wissenschaften – ISAS – e.V., Dortmund, Germany
contact email	yu-heng.hsieh@isas.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD052747
     1. Label: PRIDE project
     2. Name: Aspartate signaling increases the aggressiveness of lung metastases by inducing eIF5A-mediated translation