PXD052728

PXD052728 is an original dataset announced via ProteomeXchange.

Title	in vivo-like scaffold-free 3D in vitro Models of Muscular Dystrophies: The Case for Anchored Cell Sheet Engineering in Personalized Medicine
Description	The study of muscle dystrophies, including Duchenne and Myotonic dystrophies, has faced significant challenges due to the lack of physiologically relevant in vitro models. This research introduces a novel, scaffold- and biomaterial-free platform based on cell sheet engineering to create in vitro models using patient-specific cells. This innovative approach, known as anchored cell sheet engineering, offers a comprehensive biofabrication method that replicates mature cell phenotypes and tissue-specific extracellular matrix (ECM) deposited by the cells themselves. Primary cells from both healthy individuals and patients with Duchenne and Myotonic dystrophy type 1 were used to develop robust anchored 3D muscle fibers. The models were extensively characterized using proteomics analysis revealing that they form mature constructs closely resembling in vivo conditions, surpassing traditional 2D cultures in translational potential. Disease-specific models effectively captured key phenotypic features of the respective dystrophies. Additionally, therapeutic treatments led to significant proteomic profile changes, documented using custom Python scripts. This scaffold-free 3D modeling technique offers a promising platform for advancing muscle dystrophy research and improving drug screening methodologies for developing new treatments for complex diseases with better translatability for clinical applications.
HostingRepository	PRIDE
AnnounceDate	2024-12-24
AnnouncementXML	Submission_2024-12-24_06:35:43.100.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alireza Shahin-Shamsabadi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-30 13:25:15	ID requested
⏵ 1	2024-12-24 06:35:43	announced

10.1002/ADHM.202404465;

submitter keyword: Drug treatment, in vitro model, Cell sheet engineering, Scaffold-free, Proteomics, Dystrophy,Skeletal muscle

Alireza Shahin-Shamsbaadi
contact affiliation	Evolved.Bio, 280 Joseph Street, Kitchener, Ontario, Canada
contact email	alireza@itsevolved.com
lab head
Alireza Shahin-Shamsabadi
contact affiliation	Evolved.Bio, 280 Joseph Street, Kitchener, Ontario, Canada
contact email	alireza@itsevolved.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/12/PXD052728

PRIDE project URI

• PRIDE
  1. PXD052728
     1. Label: PRIDE project
     2. Name: in vivo-like scaffold-free 3D in vitro Models of Muscular Dystrophies: The Case for Anchored Cell Sheet Engineering in Personalized Medicine