PXD052722

PXD052722 is an original dataset announced via ProteomeXchange.

Title	DNAAF9 interactome from human airway epithelial cells
Description	Biogenesis of motile cilia in multiciliated cells (MCCs) is coupled to large-scale biosynthesis of force generating dynein motors. Shulin inhibits pre-assembled ODA (outer dynein arm) motors by packaging them into a closed conformation preventing aberrant off-target interactions with cellular microtubules in multiciliated Tetrahymena cells and co-localises with them in growing cilia. How packaged ODAs engage the Intraflagellar Transport machinery (IFT) to transit from the cytoplasm into growing cilia remains unclear. The mechanism of Shulin’s release for ODA activation inside cilia is also poorly resolved. Using proteomics, we establish links between DNAAF9 (human Shulin) and mammalian ODAs, the anterograde IFT-B complex and a ciliary small GTPase called ARL3. Based on our findings, we propose that Shulin/DNAAF9 enforces ODA inhibition to aid their unimpeded trafficking from the cytoplasm into growing multicilia via IFT and once inside the ciliary compartment, ARL3-GTP competes for a binding site to destabilise the ODA-Shulin complex thereby promoting ODA motor activation.
HostingRepository	PRIDE
AnnounceDate	2025-07-31
AnnouncementXML	Submission_2025-07-31_09:40:30.987.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Girish Mali
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-30 09:16:37	ID requested
⏵ 1	2025-07-31 09:40:31	announced

10.21203/RS.3.RS-5233849/V1;

submitter keyword: LC-MSMS, Axonemal dyneins, DNAAF, TMT,Human multiciliated airway cells

Girish Ram Mali
contact affiliation	Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE
contact email	girish.mali@path.ox.ac.uk
lab head
Girish Mali
contact affiliation	Sir William Dunn School of Pathology, University of Oxford
contact email	girish.mali@path.ox.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD052722

PRIDE project URI

• PRIDE
  1. PXD052722
     1. Label: PRIDE project
     2. Name: DNAAF9 interactome from human airway epithelial cells