PXD052687

PXD052687 is an original dataset announced via ProteomeXchange.

Title	Human OTX2 TurboID Analysis in Transfected HDMB03 Cells
Description	HDMB03 cells were transiently transfected with plasmids expressing NLS-TurboID or 3xHA-TurboID-OTX2 under the control of a strong CAG promoter. After a 15-minute pulse with 500 µM exogenous biotin, RIPA lysates were prepared and processed with streptavin-sepharose to capture biotinylated proteins that were identified by using mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2024-05-31
AnnouncementXML	Submission_2024-05-31_08:33:46.620.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Brad Doble
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotinylated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-29 14:58:00	ID requested
⏵ 1	2024-05-31 08:33:47	announced

Dataset with its publication pending

submitter keyword: Human, Medulloblastoma, TurboID, HDMB03 Cells, OTX2, Proximity Labeling

Tamra Erin Ogilvie
contact affiliation	Department of Pediatrics, Section of Hematology-Oncology Baylor College of Medicine Texas Children's Hospital Houston, Texas
contact email	tamra.ogilvie@bcm.edu
lab head
Brad Doble
contact affiliation	University of Manitoba
contact email	brad.doble@umanitoba.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD052687

PRIDE project URI

• PRIDE
  1. PXD052687
     1. Label: PRIDE project
     2. Name: Human OTX2 TurboID Analysis in Transfected HDMB03 Cells