PXD052684

PXD052684 is an original dataset announced via ProteomeXchange.

Title	PUS7 cytoplasmic localization directs a pseudouridine-mediated cellular stress response
Description	Pseudouridine (Ψ) is an abundant post-transcriptional modification found across all classes of RNA. Since its discovery in mRNAs a decade ago, it has been widely speculated that Y might govern additional post-transcriptional regulation of gene expression. Here, we demonstrate that one of the principal enzymes responsible for adding Y to mRNAs, pseudouridine synthase 7 (PUS7), accumulates in the cytoplasm under a variety of stress conditions in Saccharomyces cerevisiae and BEAS-2B human epithelial lung cells. The localization of PUS7 to the cytoplasm promotes Y-incorporation into hundreds of different mRNA sequences and increases cellular fitness under ROS and divalent metal ion stress. The preponderance of newly identified Y-sites lies within portions of the mRNA important for post-transcriptional control—-coding regions and 3’ UTRs. Quantitative proteomics reveal that shifts in the cellular post-transcriptional modification landscape upon PUS7 relocalization reshapes the proteome. Our data suggest a mechanism whereby stressors localize PUS7 in the cytoplasm to enable the direct modification and regulation of stress response mRNAs, thereby protecting cells from further stress-induced damage. (2A-PUS7-control 68283 2B-PUS7-control 68284 2C-PUS7-control 68285 4A-PUS7-NES-Co 68286 4A-PUS7-NES-Co 68287 4B-PUS7-NES-Co 68288 5A-PUS7-control-Co 68289 5B-PUS7-control-Co 68290 5C-PUS7-control-Co 68291 PUS7-NES_CU_1A 75059 PUS7-NES_CU_1B 75060 PUS7-NES_CU_1C 75061 PUS7-NLS_CU_2A 75062 PUS7-NLS_CU_2B 75063 PUS7-NLS_CU_2C 75064 PUS7-WT_CU_3A 75065 PUS7-WT_CU_3B 75066 PUS7-WT_CU_3C 75067)
HostingRepository	PRIDE
AnnounceDate	2026-01-28
AnnouncementXML	Submission_2026-01-28_01:51:00.758.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Minli Ruan
SpeciesList	scientific name: Saccharomyces cerevisiae BY4741; NCBI TaxID: NEWT:1247190;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-29 14:30:23	ID requested
⏵ 1	2026-01-28 01:51:01	announced

Dataset with its publication pending

submitter keyword: None

Kristin Koutmou
contact affiliation	Associate Professor of Chemistry Dow Early Career Professor of Chemistry Associate Director of the Program in Chemical Biology Co-Director the Chemical Biology Interface Training Program University of Michigan 930 N University Ann Arbor, MI 48109
contact email	kkoutmou@umich.edu
lab head
Minli Ruan
contact affiliation	University of Michigan
contact email	mlruan@umich.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD052684

PRIDE project URI

• PRIDE
  1. PXD052684
     1. Label: PRIDE project
     2. Name: PUS7 cytoplasmic localization directs a pseudouridine-mediated cellular stress response