PXD052681

PXD052681 is an original dataset announced via ProteomeXchange.

Title	Physicochemical and proteomic characterization reveals a remodeling of Escherichia coli and Proteus mirabilis outer membrane vesicles obtained under culture conditions mimicking urine growth
Description	Uropathogenic bacteria present a variety of mechanisms that allow them to colonize the urinary tract. These mechanisms include biofilm formation, urothelial cell invasion, and the production of adhesins, toxins, and siderophores. Uropathogenic Escherichia coli and Proteus mirabilis are two of the most common etiological agents causing urinary tract infections (UTI). In addition to virulence factors, Gram-negative bacteria can produce outer membrane vesicles (OMVs). The OMVs may have several functions in the context of UTI. In the present work, we isolated and characterized OMVs from two clinical strains: E. coli U144 and P. mirabilis 2921 cultured in Luria-Bertani broth and artificial urine. The OMVs were examined using DLS, NTA and TEM and found to be between 85 and 260 nm in size, the largest being those obtained in artificial urine. All OMVs had a low polydispersity factor and negative charge in their surface. Through proteomic analysis (nanoLC-MS/MS), 282 and 353 proteins were identified in OMVs obtained from E. coli and P. mirabilis LB cultures respectively, and 215 and 103 proteins when they were grown in AU. The majority of proteins were from the bacterial envelope. Also, several proteins related to motility and adhesion were found. The composition of OMVs proteins was different according to the culture medium. Among the identified proteins, those related with zinc and iron uptake systems could have an important role in AU. These results bring us closer to understanding the possible role of OMVs in the pathogenesis of UTIs.
HostingRepository	PRIDE
AnnounceDate	2024-10-25
AnnouncementXML	Submission_2024-10-25_05:55:29.793.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Analía Lima
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Proteus mirabilis; NCBI TaxID: 584;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-29 12:25:51	ID requested
⏵ 1	2024-10-25 05:55:30	announced

10.3389/FMICB.2024.1493859;

submitter keyword: shotgun proteomics,Escherichia coli, Proteus mirabilis, outer membrane vesciles

Paola Scavone
contact affiliation	Laboratorio de Biofilms Microbianos, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable
contact email	pscavone@gmail.com
lab head
Analía Lima
contact affiliation	Institut Pasteur de Montevideo, Proteomic and Analytical Biochemistry Unit
contact email	alima@pasteur.edu.uy
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD052681
     1. Label: PRIDE project
     2. Name: Physicochemical and proteomic characterization reveals a remodeling of Escherichia coli and Proteus mirabilis outer membrane vesicles obtained under culture conditions mimicking urine growth