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PXD052665

PXD052665 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic analysis of the major alkali-soluble Sacha Inchi (Plukenetia volubilis) proteins
DescriptionSacha Inchi (Plukenetia volubilis) oil press-cake (SIPC) represents a new source of proteins of high biological value, with promissory food applications. However, knowledge of these proteins remains limited. In this study, a Sacha Inchi protein concentrate (SPC) was extracted from the SIPC, and proteomic analysis was performed to identify the major alkaline-soluble proteins. Electrophoretic profiles highlighted the efficacy of conditions to extract the major proteins, from which five proteins, not previously reported, were registered. LC-MS/MS analyses produced abundant high-quality fragmentation spectra. Utilizing the Euphorbiaceae database (DB), 226 proteins were identified, with numerous well-assigned spectra remaining unidentified. The PEAKS Studio software generated high quality de novo peptides. Gene ontology (GO) classification allowed the identification of sequenced proteins associated with biological processes, molecular functions, and cellular components. The main alkali-soluble proteins from SPC were characterized by the derived functional analysis like seed storage, defense-related, and carbohydrate and lipid metabolism-related proteins. These results contribute to the information of the emerging proteomic studies on Sacha Inchi.
HostingRepositoryPRIDE
AnnounceDate2025-05-07
AnnouncementXMLSubmission_2025-05-06_17:31:22.013.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD052665
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterBlanca Hernández-Ledesma
SpeciesList scientific name: Plukenetia volubilis; NCBI TaxID: 316893;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-05-29 02:25:55ID requested
12025-05-06 17:31:22announced
Publication List
10.6019/PXD052665;
10.3390/foods13203275;
Torres-S, á, nchez E, Morato E, Hern, á, ndez-Ledesma B, Guti, é, rrez LF, ) Proteins. Foods, 13(20):(2024) [pubmed]
Keyword List
submitter keyword: Sachi Inchi
alkali-soluble proteins
SDS-PAGE
proteome
Blast2GO
PEAKS.
Contact List
Blanca Hernández-Ledesma
contact affiliationInstituto de Investigación en Ciencias de la Alimentación (CIAL, CSIC-UAM, CEI- UAM+CSIC), Nicolás Cabrera 9, 28049, Madrid, Spain
contact emailb.hernandez@csic.es
lab head
Blanca Hernández-Ledesma
contact affiliationInstitute of Food Science Research (CIAL, CSIC-UAM)
contact emailb.hernandez@csic.es
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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