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PXD052663

PXD052663 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAn 8-Mercaptoquinoline Motif Enriches Minichromosome Maintenance Complex Components as Significant Metalloprotein Targets in Live Cells
DescriptionAffinity-based probes are valuable tools for detecting binding interactions between small molecules and proteins in complex biological environments. Metalloproteins are a class of therapeutically significant biomolecules which bind metal ions as part of key structural or catalytic domains and are compelling targets for study. However, there is currently a limited range of chemical tools suitable for profiling the metalloproteome. Here, we describe the preparation and application of a novel, photoactivatable affinity based probe for detection of a subset of previously challenging to engage metalloproteins. The probe, bearing an 8 mercaptoquinoline metal chelator, was anticipated to engage several zinc metalloproteins, including the 26S proteasome subunit Rpn11. Upon translation of the labelling experiment to mammalian cell lysates, proteomic analysis revealed that several metalloproteins were competitively enriched. An 8-mercaptoquinoline diazirine probe was found to effectively enrich multiple components of the minichromosome maintenance complex, a zinc metalloprotein assembly with helicase activity essential to DNA replication.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:47:49.596.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSean McKenna
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-05-29 02:07:45ID requested
12024-06-27 22:11:02announced
22024-10-22 06:47:50announced2024-10-22: Updated project metadata.
Publication List
10.1039/D4CB00053F;
Keyword List
submitter keyword: photoaffinity labelling, affinity-based protein profiling, metalloenzymes,Affinity-based probes, metalloproteins
Contact List
Professor Joanna F. McGouran
contact affiliationSchuler Assistant Professor In Translational Organic Chemistry, School of Chemistry, Trinity College Dublin, Republic of Ireland
contact emailjmcgoura@tcd.ie
lab head
Sean McKenna
contact affiliationTrinity College Dublin Leiden University
contact emailmckenns8@tcd.ie
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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