PXD052626

PXD052626 is an original dataset announced via ProteomeXchange.

Title	FACS-sorted CD103+ and CD103- Treg cell proteomics
Description	Regulatory T cells (Tregs) play a key role in suppressing systemic effector immune responses, thereby preventing autoimmune diseases but could also potentially contribute to tumor progression. As a result, there is significant interest in the clinical manipulation of Tregs. However, the mechanisms governing induced Treg (iTreg) differentiation are not fully understood. In the present study, we phenotypically profiled human iTregs during early stages of in vitro differentiation at single-cell level using multiparametric mass cytometry. A panel of 25 metal-conjugated antibodies specific to a range of markers associated with human Tregs was used to characterize these immunomodulatory cells. We found that iTregs highly express the transcription factor Foxp3 and characteristic Treg-associated surface markers (e.g. CD25, PD1, CD137, CCR4, CCR7, CXCR3, and CD103). Expression of co-inhibitory factors (e.g. TIM3, LAG3, and TIGIT) increased slightly at late stages of iTreg differentiation. Further, CD103 was selectively upregulated in the iTreg compartment while negatively regulated by Foxp3. Overall, our study highlights that during early stages of differentiation, iTregs resemble memory-like Treg features, and opens possibilities for studying molecular mechanisms of Treg function.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-06_17:39:55.781.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kedar Batkulwar
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-28 04:52:38	ID requested
⏵ 1	2025-05-06 17:39:56	announced

10.1007/s00018-024-05429-3;

Kattelus R, Starskaia I, Lind, é, n M, Batkulwar K, Pietil, ä S, Moulder R, Marson A, Rasool O, Suomi T, Elo LL, Lahesmaa R, Buchacher T, Phenotypic profiling of human induced regulatory T cells at early differentiation: insights into distinct immunosuppressive potential. Cell Mol Life Sci, 81(1):399(2024) [pubmed]

submitter keyword: FAIMS, DIA, FOXP3, Treg, T cell differentiation,CD103

Riitta Lahesmaa
contact affiliation	Molecular Systems Immunology Group Turku Bioscience University of Turku P.O. Box 123, BioCity (Street adr. Tykistökatu 6 A, 7th floor) FIN-20521 Turku, Finland +358-40-718 4813 (Mobile)
contact email	rilahes@utu.fi
lab head
Kedar Batkulwar
contact affiliation	Turku Bioscience, University of Turku, Turku
contact email	kebatk@utu.fi
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD052626

PRIDE project URI

• PRIDE
  1. PXD052626
     1. Label: PRIDE project
     2. Name: FACS-sorted CD103+ and CD103- Treg cell proteomics