PXD052602

PXD052602 is an original dataset announced via ProteomeXchange.

Title	Proteomic investigation of human dental pulp to identify individuals who are pregnant
Description	Biomolecules preserved in dental pulp are increasingly being used to identify individuals in the context of forensics and archaeology. Despite the vast amount of research into host and pathogen DNA, the potential use of physiologically informative proteins preserved in dental pulp has rarely been studied. Here, we hypothesized that pregnancy specific proteins circulating in the blood could be identified from the dental pulp of postpartum individuals and this was investigated using 8 human third molars extracted from 4 postpartum and 3 control individuals during clinical treatment. A total of 885 proteins were identified from these 8 dental pulp samples using liquid chromatography coupled tandem mass spectrometry, whose gene ontology compositions were similar to previous studies. However, despite our hypothesis, pregnancy specific proteins were not identified from the dental pulp of postpartum individuals (n = 5, 4–12 months postpartum). Although the dental pulp proteomes obtained from three individuals postpartum ≤6 months were distinct from those of other individuals by principal component analysis, their driving proteins were less evident. Although our hypothesis was not supported, sample collection, protein extraction, and mass spectrometry analysis could be improved to explore the forensic application of detecting pregnancy specific proteins in dental pulp.
HostingRepository	PRIDE
AnnounceDate	2025-05-24
AnnouncementXML	Submission_2025-05-23_16:01:01.804.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Takumi Tsutaya
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-27 16:59:03	ID requested
⏵ 1	2025-05-23 16:01:02	announced

10.1002/PRCA.70011;

submitter keyword: Postpartum, Blood circulation, Forensic identification, Pregnancy,Dental pulp

Takumi Tsutaya
contact affiliation	Research Center for Integrative Evolutionary Science, The Graduate University for Advanced Studies (SOKENDAI), Japan
contact email	tsutayatakumi@gmail.com
lab head
Takumi Tsutaya
contact affiliation	The Graduate University for Advanced Studies
contact email	tsuta16@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD052602
     1. Label: PRIDE project
     2. Name: Proteomic investigation of human dental pulp to identify individuals who are pregnant