PXD052599

PXD052599 is an original dataset announced via ProteomeXchange.

Title	Identification of putative phosphorylation substrates of BRI1 in response to brassinolide and sucrose
Description	Plants respond to various external and internal stimuli by activating signal transduction pathways. The plasma membrane, acting as the cell boundary, provides a platform for various signaling components. These include cell surface receptor-like kinases, intrinsic transporters, membrane-anchored proteins, and cytosolic signaling components attached to the membrane from its cytosolic side. Phosphorylation is one of the most sensitive and essential post-translational modifications. To identify the sequential kinase-target relationships and understand the triggers for downstream regulatory complexes, we employed a phosphoproteomic approach. By subjecting plants to brassinolide and/or sucrose, we aim to identify the BR-BRI1 downstream substrates and investigate the possible impact of sucrose on this signaling pathway.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-06_17:39:19.164.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lin Xi
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	phosphorylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-27 12:35:22	ID requested
⏵ 1	2025-05-06 17:39:19	announced

Xi L, Wu X, Wang J, Zhang Z, He M, Zeeshan Z, Stefan T, Schulze WX, Receptor Kinase Signaling of BRI1 and SIRK1 Is Tightly Balanced by Their Interactomes as Revealed From Domain-Swap Chimaera in AE-MS Approaches. Mol Cell Proteomics, 23(11):100857(2024) [pubmed]

10.1016/j.mcpro.2024.100857;

submitter keyword: sucrose, phosphorylaion, kinase-target,branssinolide, plasma membrane

Waltraud Schulze
contact affiliation	Department of Plant Systems Biology, University of Hohenheim, 70599 Stuttgart, Germany
contact email	wschulze@uni-hohenheim.de
lab head
Lin Xi
contact affiliation	University of Hohenheim
contact email	lin.xi.260@uni-hohenheim.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD052599
     1. Label: PRIDE project
     2. Name: Identification of putative phosphorylation substrates of BRI1 in response to brassinolide and sucrose