PXD052570

PXD052570 is an original dataset announced via ProteomeXchange.

Title	diaPASEF and DIA of proteome and phosphoproteome of VSV-stimulated THP1 cells
Description	Infection of mammalian cells by vesicular stomatitis virus (VSV) triggers the activation of the innate immunity pathway. VSV RNA is recognized by RIG-I, leading to the activation of RIG-I and subsequent activation of the essential effector MAVS. To gain a comprehensive understanding of the molecular events occurring in VSV-infected THP1 cells, we employed data-independent acquisition (DIA) and diaPASEF techniques to profile the dynamic phosphoproteomes and proteomes of wild-type (WT), RIG-I knockout (KO), and MAVS KO THP1 cells. This approach enabled the quantification of approximately 8000 proteins and 11000 localized phosphopeptides. The in-depth proteomic and phosphoproteomic analyses provided insights into the molecular mechanisms underlying VSV-induced ATM-mediated PBX2 degradation. Moreover, the phosphoproteomic data revealed that VSV induces phosphorylation events in THP1 cells independently of RIG-I. Additionally, we identified five phosphosites on VSV-coding proteins and predicted the corresponding kinases in host cells. Overall, our findings offer a comprehensive proteomic and phosphoproteomic map of VSV stimulation, highlighting RIG-I or MAVS-related phosphorylation events. This resource serves as a valuable foundation for future studies aimed at understanding the RNA-sensing innate immunity signaling pathway.
HostingRepository	iProX
AnnounceDate	2024-05-27
AnnouncementXML	Submission_2024-05-26_19:26:37.163.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chuanqi Zhong
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion Lumos; timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-26 19:25:27	ID requested
⏵ 1	2024-05-26 19:26:37	announced

Dataset with its publication pending

submitter keyword: VSV, phosphoproteome

Chuanqi Zhong
contact affiliation	Xiamen University
contact email	zhongcq@xmu.edu.cn
lab head
Chuanqi Zhong
contact affiliation	Xiamen University
contact email	zhongcq@xmu.edu.cn
dataset submitter

iProX dataset URI