PXD052567

PXD052567 is an original dataset announced via ProteomeXchange.

Title	Campylobacter rsmA mutantIncreased motility in Campylobacter jejuni and changes in its virulence, fitness and morphology following protein expression on ribosomes with altered RsmA methylation
Description	Infection with Campylobacter jejuni is the major cause of human gastroenteritis in the United States and Europe, leading in many cases to debilitating autoimmune sequelae. While considerable progress has been made in detailing the infectious cycle of C. jejuni, a full understanding of the molecular mechanisms responsible for virulence remains to be elucidated. Here, we apply a novel approach by modulating protein expression on the pathogen’s ribosomes by inactivating a highly conserved rRNA methyltransferase. Loss of the RsmA methyltransferase results in a more motile strain with greater adhesive and cell invasive properties. These phenotypical effects correlate with enhanced expression of specific proteins related to flagellar formation and function, together with enzymes involved in cell wall/membrane and amino acid synthesis. Despite the enhancement of certain virulent traits, the null-strain grows poorly on minimal media and is rapidly out-competed by the wild-type strain. Complementation with an active copy of the rsmA gene rescues most of the traits changed in the mutant. However, the complemented strain overexpresses rsmA and displays new flaws including loss of the spiral cell shape that is distinctive for C. jejuni. Proteins linked with altered virulence and morphology are identified here by mass spectrometry proteomic analyses of the strains.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-06_17:54:55.661.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Agnieszka Guz
SpeciesList	scientific name: Campylobacter jejuni subsp. jejuni 81-176; NCBI TaxID: 354242; scientific name: Bacteria; NCBI TaxID: NCBITaxon:2;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-26 11:24:17	ID requested
⏵ 1	2025-05-06 17:54:56	announced

10.3390/ijms25189797;

Sa, ł, amaszy, ń, ska-Guz A, Murawska M, B, ą, cal P, Ostrowska A, Kwiecie, ń E, Stefa, ń, ska I, Douthwaite S, and Changes in Its Virulence, Fitness, and Morphology Following Protein Expression on Ribosomes with Altered RsmA Methylation. Int J Mol Sci, 25(18):(2024) [pubmed]

submitter keyword: methyltransferases,Campylobacter

Agnieszka Sałamaszyńska-Guz
contact affiliation	Institute of Veterinary Medicine Warsaw University of Life Sciences
contact email	agnieszka_salamaszynska_guz@sggw.edu.pl
lab head
Agnieszka Guz
contact affiliation	Faculty of Veterinary Sciences; Warsaw University of Life Sciences;
contact email	asalam@tlen.pl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD052567
     1. Label: PRIDE project
     2. Name: Campylobacter rsmA mutantIncreased motility in Campylobacter jejuni and changes in its virulence, fitness and morphology following protein expression on ribosomes with altered RsmA methylation