PXD052566

PXD052566 is an original dataset announced via ProteomeXchange.

Title	PC4-IP complex in Huh7 cells during G1 and G2 phases
Description	Numerous genes exhibit periodic oscillations in mRNA expression, essential for orderly cell division. Mitosis-related mRNAs fluctuate cyclically from the G2 to M phase, primarily regulated by transcription factors. However, the role of post-transcriptional regulation in this process remains unclear. Here, we demonstrated a decrease in mRNA levels of centromere protein F (CENPF) from the early to late G2 phase. Positive cofactor 4 (PC4) serves as a crucial post-transcriptional regulator, orchestrating the periodic degradation of CENPF mRNA, ensuring balanced CENP expression, proper spindle assembly, and successful mitosis. In early G2, newly synthesised CENPF mRNAs accumulate and bind to PC4, leading to SETDB1-mediated PC4 dimethylation at K35. In late G2, dimethylated PC4 interacts with UPF1 to promote deadenylation-dependent degradation of CENPF mRNAs, forming a regulatory loop for CENP homeostasis. Elevated PC4 dimethylation in hepatocellular carcinoma, coupled with increased sensitivity to taxanes upon its inhibition, suggests promising therapeutic avenues. These findings suggest a post-transcriptional quality control mechanism regulating cyclic mitotic mRNA fluctuations, providing comprehensive insights into cell cycle gene regulation dynamics.
HostingRepository	PRIDE
AnnounceDate	2025-01-31
AnnouncementXML	Submission_2025-01-30_18:11:39.933.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qimei Pan
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-26 00:13:53	ID requested
⏵ 1	2025-01-30 18:11:40	announced

Dataset with its publication pending

submitter keyword: PC4, Huh7, G2 phase, G1 phase

Chunmeng Shi
contact affiliation	State Key Laboratory of Trauma and Chemical Poisoning, Third Military Medical University, Chongqing, China
contact email	shicm@tmmu.edu.cn
lab head
Qimei Pan
contact affiliation	Army Medical University
contact email	mayble_pan@sina.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/01/PXD052566

PRIDE project URI

• PRIDE
  1. PXD052566
     1. Label: PRIDE project
     2. Name: PC4-IP complex in Huh7 cells during G1 and G2 phases