PXD052559

PXD052559 is an original dataset announced via ProteomeXchange.

Title	Characterization of Exosomes Released from Mycobacterium abscessus-infected Macrophages
Description	Extracellular vesicles, such as exosomes, play a critical role in cell-to-cell communication and have been found to modulate cellular processes, including metabolic and inflammatory pathways, in recipient cells. Non-tuberculous Mycobacteria (NTM), such as Mycobacterium abscessus (M.ab), are a group of environmental bacteria that can cause severe lung infections in populations with pre-existing lung conditions, such as cystic fibrosis (CF) and chronic obstructive pulmonary disease (COPD). There is limited knowledge of the engagement of extracellular vesicles in the host-pathogen interactions in the context of NTM infections. In this study, we found that M.ab infection increased the release of a subpopulation of exosomes (CD9, CD63 and/or CD81 positive) by mouse macrophages but did not affect exosome morphology. Proteomic analysis of the vesicles demonstrated that M.ab infection affects the enrichment of host proteins in exosomes released by macrophages. When compared to exosomes from uninfected macrophages, exosomes released by M.ab-infected macrophages significantly improved M.ab growth and downregulated the intracellular level of glutamine in recipient macrophages in cell culture. Interestingly, the protein abundance of the glutamine transporters Slc1a5 and Slc38a2 were found to be enriched in exosomes from M.ab-infected macrophages compared to those from uninfected macrophages. Increasing glutamine concentration in the medium rescued intracellular glutamine levels and inhibited M.ab growth in recipient macrophages treated with exosomes from M.ab-infected macrophages. Taken together, our results indicate that exosomes may serve as extracellular glutamine eliminators that interfere with glutamine-dependent M.ab killing in recipient macrophages. This suggests that the development of exosome-targeting therapies may have the potential to augment current treatments for mycobacterial infections.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-06_17:34:50.905.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Steven Hartson
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Mycobacterium abscessus (strain ATCC 19977 / DSM 44196 / CIP 104536 / JCM 13569 / NCTC 13031 / TMC 1543); NCBI TaxID: 561007;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-24 15:33:56	ID requested
⏵ 1	2025-05-06 17:34:51	announced

10.1002/pmic.202400181;

Vermeire CA, Tan X, Ramos-Leyva A, Wood A, Kotey SK, Hartson SD, Liang Y, Liu L, Cheng Y, Characterization of Exosomes Released from Mycobacterium abscessus-Infected Macrophages. Proteomics, 25(3):e202400181(2025) [pubmed]

submitter keyword: exosomes, macrophages,Mycobacterium abscessus

Yong Cheng
contact affiliation	Department of Biochemistry and Molecular Biology, Oklahoma Center for Respiratory and Infectious Diseases, Oklahoma State University, Stillwater, Oklahoma, USA
contact email	ycheng@okstate.edu
lab head
Steven Hartson
contact affiliation	Oklahoma State University
contact email	hartson.steve@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD052559

PRIDE project URI

• PRIDE
  1. PXD052559
     1. Label: PRIDE project
     2. Name: Characterization of Exosomes Released from Mycobacterium abscessus-infected Macrophages