PXD052553

PXD052553 is an original dataset announced via ProteomeXchange.

Title	Bacterial EVs as intranasal postbiotics: Detailed characterization and interaction with airway cells
Description	Escherichia coli A0 34/86 (EcO83) is a probiotic bacterium used in newborns to prevent nosocomial infections and diarrhoea. Mechanistically, EcO83 induces the production of pro- and anti-inflammatory cytokines in vitro and in vivo and, when administered intranasally, inhibits allergic airway inflammation in mice by interacting with innate pattern recognition receptors. Despite the proven therapeutic benefit, there is a concern about the use of live bacteria due to the risk of systemic infections and bacterial gene transfer. Therefore, EcO83-derived extracellular vesicles (EcO83-EVs) could represent a safer alternative to live bacteria. However, the exact mechanism of interaction between EV and host remains to be elucidated. Here, we have isolated, purified, and characterised EcO83-EVs following the Minimal Information of Studies of Extracellular Vesicles (MISEV) guidelines. Our ex vivo studies in human nasal epithelial cells showed that EcO83-EVs increased the expression of proteins linked to oxidative stress and inflammation, indicating an effective interaction between EVs and the host cells. Further in vivo studies in mice demonstrated that EcO83-EVs interact with nasal-associated lymphoid tissue, are internalised by airway macrophages, and stimulate neutrophil recruitment in the lung. In vitro analyses revealed that EcO83-EVs activate the NF-κΒ signalling pathway, resulting in the nitric oxide production. Our findings suggest that EcO83-EVs could be an effective and safer postbiotic alternative to live bacteria. Further research is needed to explore the potential of EVs from probiotic bacteria for clinical applications, particularly for mucosal vaccines or targeted immunotherapies.
HostingRepository	PRIDE
AnnounceDate	2024-11-06
AnnouncementXML	Submission_2024-11-06_00:36:51.561.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Akos Vegvari
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-24 05:27:08	ID requested
⏵ 1	2024-11-06 00:37:10	announced

10.1002/jev2.70004;

Razim A, Zab, ł, ocka A, Schmid A, Thaler M, Č, ern, ý V, Weinmayer T, Whitehead B, Martens A, Skalska M, Morandi M, Schmidt K, Wysmo, ł, ek ME, V, é, gv, á, ri A, Srutkova D, Schwarzer M, Neuninger L, Nejsum P, Hrd, ý J, Palmfeldt J, Brucale M, Valle F, G, ó, rska S, Wisgrill L, Inic-Kanada A, Wiedermann U, Schabussova I, Bacterial extracellular vesicles as intranasal postbiotics: Detailed characterization and interaction with airway cells. J Extracell Vesicles, 13(10):e70004(2024) [pubmed]

submitter keyword: macrophage, postbiotic, nitric oxide, NF-κΒ signalling, probiotic,bacterial extracellular vesicles

Akos Vegvari
contact affiliation	Karolinska Institutet
contact email	akos.vegvari@ki.se
lab head
Akos Vegvari
contact affiliation	Karolinska Institutet
contact email	akos.vegvari@ki.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/11/PXD052553

PRIDE project URI

• PRIDE
  1. PXD052553
     1. Label: PRIDE project
     2. Name: Bacterial EVs as intranasal postbiotics: Detailed characterization and interaction with airway cells