PXD052552

PXD052552 is an original dataset announced via ProteomeXchange.

Title	A photo-caged cross-linker for identifying protein-protein interactions
Description	In recent years cross-linking mass spectrometry (XL-MS) as a tool to study protein-protein interactions (PPIs) has undergone significant improvements, which were mainly propelled by the emergence of novel crosslinkers and the development and implementation of advanced analysis workflows. Despite these recent advancements, addressing PPIs in the cell by XL-MS remains a substantial challenge. Some of the current limitations are linked to a comparable poor membrane permeability of most crosslinkers and their limited efficiency to traverse the cellular membrane. To address this problem, we have designed and synthesized a novel crosslinker based on the chemical o-nitrobenzylester (o-NBE) group, which enhances the hydrophobic properties of the cross-linker.
HostingRepository	PRIDE
AnnounceDate	2024-11-21
AnnouncementXML	Submission_2024-11-21_03:52:57.490.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Florian Stengel
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetic acid derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-24 05:19:26	ID requested
⏵ 1	2024-11-21 03:52:57	announced

10.1002/CBIC.202400620;

submitter keyword: proteomics, mass spectrometry,Cross-linker, protein-protein interactions

Florain Stengel
contact affiliation	Prof. Dr. Florian Stengel,Department of Biology, University of Konstanz
contact email	florian.stengel@uni-konstanz.de
lab head
Florian Stengel
contact affiliation	Universität Konstanz
contact email	florian.stengel@uni-konstanz.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/11/PXD052552

PRIDE project URI

• PRIDE
  1. PXD052552
     1. Label: PRIDE project
     2. Name: A photo-caged cross-linker for identifying protein-protein interactions