PXD052543

PXD052543 is an original dataset announced via ProteomeXchange.

Title	Chicken embryonic stem cell/primordial germ cell LC-MSMS
Description	As germ cell precursor, primordial germ cells (PGCs) are widely used in transgenic animal production, regenerative medicine and other fields. However, the regulation mechanism of chicken PGCs is not incomplete, which leads to the insufficient amount of chicken PGCs obtained in vitro, which seriously affects the specific application of PGCs. During PGC formation (differentiation from ESCs to PGCs), some proteins have inconsistent changes in transcription level and protein abundance. Mediating proteasome degradation is one of the most important roles of protein ubiquitination, and enrichment analysis of transcriptome and proteome also suggests an important role of ubiquitination in the process of PGCs. In order to explore the important functions and potential targets of ubiquitination, we collected chicken ESCs and PGCs cells for label free ubiquitomics analysis. This study preliminarily analyzed how ubiquitination regulates the formation of chicken PGCs, providing a theoretical basis for the subsequent research and specific application of PGCs.
HostingRepository	PRIDE
AnnounceDate	2025-09-08
AnnouncementXML	Submission_2025-09-07_16:09:51.078.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qisheng Zuo
SpeciesList	scientific name: Gallus gallus (Chicken); NCBI TaxID: 9031;
ModificationList	ubiquitination signature dipeptidyl lysine
Instrument	ultraflex

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-23 23:27:29	ID requested
⏵ 1	2025-09-07 16:09:52	announced

10.1093/jas/skae251;

Gong W, Liu X, Lv X, Zhang Y, Niu Y, Jin K, Li B, Zuo Q, Ubiquitination plays an important role during the formation of chicken primordial germ cells. J Anim Sci, 102():(2024) [pubmed]

submitter keyword: LC-MSMS, Primordial germ cell, Embryonic stem cell,Chicken

Qisheng Zuo
contact affiliation	1 Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu225009, P.R. China 2 Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu225009, P.R. China
contact email	006664@yzu.edu.cn
lab head
Qisheng Zuo
contact affiliation	Yangzhou University
contact email	006664@yzu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD052543

PRIDE project URI

• PRIDE
  1. PXD052543
     1. Label: PRIDE project
     2. Name: Chicken embryonic stem cell/primordial germ cell LC-MSMS