PXD052538

PXD052538 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Use LC-MS/MS to investigate the global regulation of KhpA and KhpB on protein expression in Deinococcus radiodurans
Description	D. radiodurans WT, khpA, and khpB strains were grown in TGY until the OD600 reached 0.8. Cells were collected from 10 mL of each culture and harvested by centrifugation at 4000 rpm and 4 C for 10 min. The pellet was resuspended in 500 ul of 1 PBS supplemented with 1 mM PMSF (phenylmethylsulfonyl fluoride) and lysed using a sonicator (XL-2000 Microson ultrasonic liquid processor; QSonica) for ten times of 30 s bursts with 1 min of rest on ice between each burst. Following sonication, the samples were centrifuged at 12,000 rpm and 4 C for 10 min to get the soluble protein lysate in the supernatant. The protein concentration was determined using the Bradford assay.
HostingRepository	MassIVE
AnnounceDate	2024-05-23
AnnouncementXML	Submission_2024-05-23_14:53:57.227.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Runhua Han
SpeciesList	scientific name: deinococcus radiodurans;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-23 14:31:58	ID requested
⏵ 1	2024-05-23 14:53:57	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: small RNA, RNA-protein interaction, RNA binding protein, RNA regulation Deinococcus radiodurans

submitter keyword: small RNA, RNA-protein interaction, RNA binding protein, RNA regulation Deinococcus radiodurans

Contact List

Lydia M. Contreras
contact affiliation	the University of Texas at Austin
contact email	lcontrer@che.utexas.edu
lab head
Runhua Han
contact affiliation	UT Austin
contact email	runhuahan@gmail.com
dataset submitter

Full Dataset Link List

MassIVE dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v08/MSV000094857/

MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v08/MSV000094857/