PXD052534
PXD052534 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Using MS2 affinity pulldown to identify sRNA associating proteins in the bacterium Deinococcus radiodurans |
Description | We carried out the widely used MS2 aptamer approach to identify the proteins that could potentially interact with sRNAs in D. radiodurans. The MS2 aptamer was fused to the 5 extremity of PprS/Dsr2, a well-characterized D. radiodurans sRNA . This construct is then expressed off the pRADgro plasmid in wild-type D. radiodurans at mid-exponential phase. After bacterial lysis, the crude extract was loaded into an amylose-based chromatography column previously coated with the MS2 protein fused to the maltose-binding protein. This enables the specific capture of MS2-Dsr2 and its interacting protein partners. After elution, co-purified proteins were identified by LC-MS/MS and subsequent bioinformatic analysis |
HostingRepository | MassIVE |
AnnounceDate | 2024-05-23 |
AnnouncementXML | Submission_2024-05-23_13:57:56.253.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Runhua Han |
SpeciesList | scientific name: Deinococcus radiodurans R1; NCBI TaxID: 243230; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2024-05-23 13:39:31 | ID requested | |
⏵ 1 | 2024-05-23 13:57:56 | announced |
Publication List
no publication |
Keyword List
submitter keyword: small RNA, RNA-protein interaction, RNA binding protein, RNA regulation , Deinococcus radiodurans |
Contact List
Lydia M. Contreras | |
---|---|
contact affiliation | the University of Texas at Austin |
contact email | lcontrer@che.utexas.edu |
lab head | |
Runhua Han | |
contact affiliation | UT Austin |
contact email | runhuahan@gmail.com |
dataset submitter |
Full Dataset Link List
MassIVE dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v08/MSV000094854/ |