PXD052534

PXD052534 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Using MS2 affinity pulldown to identify sRNA associating proteins in the bacterium Deinococcus radiodurans
Description	We carried out the widely used MS2 aptamer approach to identify the proteins that could potentially interact with sRNAs in D. radiodurans. The MS2 aptamer was fused to the 5 extremity of PprS/Dsr2, a well-characterized D. radiodurans sRNA . This construct is then expressed off the pRADgro plasmid in wild-type D. radiodurans at mid-exponential phase. After bacterial lysis, the crude extract was loaded into an amylose-based chromatography column previously coated with the MS2 protein fused to the maltose-binding protein. This enables the specific capture of MS2-Dsr2 and its interacting protein partners. After elution, co-purified proteins were identified by LC-MS/MS and subsequent bioinformatic analysis
HostingRepository	MassIVE
AnnounceDate	2024-05-23
AnnouncementXML	Submission_2024-05-23_13:57:56.253.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Runhua Han
SpeciesList	scientific name: Deinococcus radiodurans R1; NCBI TaxID: 243230;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-23 13:39:31	ID requested
⏵ 1	2024-05-23 13:57:56	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: small RNA, RNA-protein interaction, RNA binding protein, RNA regulation , Deinococcus radiodurans

submitter keyword: small RNA, RNA-protein interaction, RNA binding protein, RNA regulation , Deinococcus radiodurans

Contact List

Lydia M. Contreras
contact affiliation	the University of Texas at Austin
contact email	lcontrer@che.utexas.edu
lab head
Runhua Han
contact affiliation	UT Austin
contact email	runhuahan@gmail.com
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v08/MSV000094854/