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PXD052534

PXD052534 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleUsing MS2 affinity pulldown to identify sRNA associating proteins in the bacterium Deinococcus radiodurans
DescriptionWe carried out the widely used MS2 aptamer approach to identify the proteins that could potentially interact with sRNAs in D. radiodurans. The MS2 aptamer was fused to the 5 extremity of PprS/Dsr2, a well-characterized D. radiodurans sRNA . This construct is then expressed off the pRADgro plasmid in wild-type D. radiodurans at mid-exponential phase. After bacterial lysis, the crude extract was loaded into an amylose-based chromatography column previously coated with the MS2 protein fused to the maltose-binding protein. This enables the specific capture of MS2-Dsr2 and its interacting protein partners. After elution, co-purified proteins were identified by LC-MS/MS and subsequent bioinformatic analysis
HostingRepositoryMassIVE
AnnounceDate2024-05-23
AnnouncementXMLSubmission_2024-05-23_13:57:56.253.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterRunhua Han
SpeciesList scientific name: Deinococcus radiodurans R1; NCBI TaxID: 243230;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-05-23 13:39:31ID requested
12024-05-23 13:57:56announced
Publication List
no publication
Keyword List
submitter keyword: small RNA, RNA-protein interaction, RNA binding protein, RNA regulation , Deinococcus radiodurans
Contact List
Lydia M. Contreras
contact affiliationthe University of Texas at Austin
contact emaillcontrer@che.utexas.edu
lab head
Runhua Han
contact affiliationUT Austin
contact emailrunhuahan@gmail.com
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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