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PXD052510

PXD052510 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProfiling FERONIA interaction proteins by Pupylation-based interacting tagging in Arabidopsis
DescriptionFERONIA (FER) is a ubiquitious expressed and a plasma memberane-localized receptor-like protein kinase which modulate many biological functions. Elucidating the interacting proteins is the crucial step in understanding the molecular function of FER in regulating plant development. However, weak and transient interactions make identifying FER interacting proteins a challenge. Here, we adapted a novel proximity-based labelling technique, pupylation-based interaction tagging (PUP-IT), to label interacting proteins, subsequent enriched by affinity capture, and analyzed by liquid chromatography-coupled tandem mass spectrometery (LC-MS/MS) to profile FER interacting proteins. To perform a quick screen, a transient protoplasts expression system was conducted to identify interacting proteins in mesophyll cells. In addition, we generated a transgenic line that harboring a tagging substrate-inducible system and FER-ligase recombinant protein driven by a native promoter for surveying the interacting proteins in seedlings and flower organ. We introduce and prove the concept of PUP-IT tool designed to detect protein interactions of significance in plants.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:54:46.390.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterZhuoran Lin
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationListmonomethylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-05-23 03:33:52ID requested
12024-08-13 03:07:19announced
22024-10-22 06:54:46announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Arabidopsis thaliana, Orbitrap Fusion Lumos(Thermo Fisher Scientific)
Contact List
Kun-Hsiang Liu
contact affiliationNorthwest A&F University
contact emailkhliu@molbio.mgh.harvard.edu
lab head
Zhuoran Lin
contact affiliationNorthwest A&F University
contact email805772406@qq.com
dataset submitter
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Dataset FTP location
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