PXD052498

PXD052498 is an original dataset announced via ProteomeXchange.

Title	Proteomic study of localized tissue necrosis by Naja atra venom
Description	Naja atra bites frequently cause immediate and serious disease. N. atra venom contains a complex of toxic components that can cause immediate and direct harm to the patient's skin tissue. If not treated effectively, it can readily progress to widespread localized necrosis, which in severe cases may result in impairment or amputation. However, the underlying causes for this localized tissue necrosis remain unknown. This study aims to investigate the protein basis of N. atra venom -induced localized tissue necrosis at the whole organism and dynamic network levels, as well as to understand the pathogenic process of this necrosis from a molecular standpoint. Venom was injected into Bama miniature pigs to induce ulcers, and exudate samples from the wounds were collected at different points over time. This model aims to provide a means to observe the ulceration process and collect exudate for further analysis regarding the effects of the venom. Through label-free proteomics analysis, we identified all protein components present in the wound exudate. The proteomics results revealed that 1119, 1016, 938, 864, and 855 proteins were identified in the exudate at 6 hours, 12 hours, 24 hours, 36 hours, and 48 hours after snake venom injection, respectively, and a total of 431 differentially expressed proteins were screened from the proteins in the exudate at the five time periods. Bioinformatics and Parallel Reaction Monitoring (PRM) with Enzyme-linked immunosorbent assay (ELISA)were used to validate further screening, and we eventually identified S100A8, MMP-2, MIF, and IDH2 as proteins associated with local tissue necrosis. In this study, we established a Bama miniature pig model for N. atra venom injection, and performed proteomic analysis of the wound exudate, which provides important insights into the molecular pathology of snakebite-induced tissue necrosis and potential theoretical bases for clinical treatment.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:48:29.029.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	ManQi Xiao
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-22 21:13:02	ID requested
1	2024-07-03 02:04:22	announced
⏵ 2	2024-10-22 06:48:29	announced	2024-10-22: Updated project metadata.

10.1016/j.toxicon.2024.107829;

Guan Z, Xiao M, Hu S, Li Y, Mo C, Yin Y, Li R, Zhang Z, Zhang X, Liao M, Proteomic study of localized tissue necrosis by Naja atra venom. Toxicon, 247():107829(2024) [pubmed]

submitter keyword: Naja atra venom；Proteomics；Parallel Reaction Monitoring；snakebite

Ming Liao
contact affiliation	Guangxi Medical University
contact email	liaominggx@126.com
lab head
ManQi Xiao
contact affiliation	Guangxi Medical University
contact email	2645917119@qq.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD052498

PRIDE project URI

• PRIDE
  1. PXD052498
     1. Label: PRIDE project
     2. Name: Proteomic study of localized tissue necrosis by Naja atra venom