PXD052492

PXD052492 is an original dataset announced via ProteomeXchange.

Title	Mistranslating serine tRNAs in Drosophila melanogaster
Description	Transfer RNAs (tRNAs) are vital in determining the specificity of translation. Mutations in tRNAs can result in the mis-incorporation of amino acids into nascent polypeptides in a process known as mistranslation. Here, our goal was to test the impacts of different types of mistranslation in the model organism Drosophila melanogaster, as impact of mistranslation depends on the type of amino acid substitution. We created two fly lines - one expressing a serine tRNA variant with valine anticodon and the other with a serine tRNA variant with a threonine anticodon. Using mass spectrometry, we measure the amount of mistranslation at various points in fly development.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:45:03.514.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD052492
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Matthew Berg
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-22 16:59:55	ID requested
1	2024-06-15 14:01:06	announced
⏵ 2	2024-10-22 06:45:03	announced	2024-10-22: Updated project metadata.

10.6019/PXD052492;

10.1101/2024.06.11.598535;

submitter keyword: fruit flies, whole proteome mass spectrometry, mistranslation, tRNA variants, Drosophila melanogaster

Judit Villén
contact affiliation	Department of Genome Sciences, University of Washington (Seattle)
contact email	jvillen@uw.edu
lab head
Matthew Berg
contact affiliation	Department of Genome Sciences, University of Washington
contact email	mattberg@uw.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/06/PXD052492

PRIDE project URI

• PRIDE
  1. PXD052492
     1. Label: PRIDE project
     2. Name: Mistranslating serine tRNAs in Drosophila melanogaster