PXD052479

PXD052479 is an original dataset announced via ProteomeXchange.

Title	Integrin beta 1 facilitates non-enveloped hepatitis E virus cell entry through the recycling endosome
Description	Hepatitis E virus (HEV) is a major cause of acute hepatitis and mainly transmitted faecal-orally. HEV particles present in faeces are naked (nHEV), whereas those found in the blood are enveloped (eHEV) with a cell-derived lipid membrane. Despite its global health impact, the cellular life cycle of HEV remains poorly understood, particularly regarding the mechanisms of viral entry into host cells. To address this knowledge gap, we have developed a high content RNA-FISH-based imaging assay that allows, for the first time, the investigation of the entry pathways of both naked and enveloped HEV particles. Surprisingly, we obtained compelling evidence that integrin α3, previously implicated in naked HEV cell entry, is not expressed in various HEV-permissive cells. Instead, we have identified integrin β1 (ITGB1) as the key player in pairing with exchangeable α-integrins to mediate naked HEV cell entry. Our results indicate that the interaction with ITGB1 facilitates naked HEV entry through the recycling endosome. In contrast, enveloped HEV particles do not interact with ITGB1 and rather use the classical endocytic route via the early endosome. Importantly, both forms of HEV require endosomal acidification and proteolytic cleavage by lysosomal cathepsins, which ultimately results in delivery of the HEV genome to the cytoplasm.
HostingRepository	PRIDE
AnnounceDate	2025-06-06
AnnouncementXML	Submission_2025-06-06_03:25:33.062.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marcel Schilling
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-22 07:34:20	ID requested
⏵ 1	2025-06-06 03:25:33	announced

Dataset with its publication pending

submitter keyword: Liver Cell Lines

Ursula Klingmüller
contact affiliation	Division Systems Biology of Signal Transduction German Cancer Research Center (DKFZ) Im Neuenheimer Feld 280 69120 Heidelberg, Germany
contact email	u.klingmueller@dkfz-heidelberg.de
lab head
Marcel Schilling
contact affiliation	Division Systems Biology of Signal Transduction, German Cancer Research Center (DKFZ), Im Neuenheimer Feld 280, 69120 Heidelberg
contact email	m.schilling@dkfz.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/06/PXD052479

PRIDE project URI

• PRIDE
  1. PXD052479
     1. Label: PRIDE project
     2. Name: Integrin beta 1 facilitates non-enveloped hepatitis E virus cell entry through the recycling endosome