PXD052475

PXD052475 is an original dataset announced via ProteomeXchange.

Title	C. albicans cells exhibit specific proteomic profiles depending on the medium used to induce the filamentation process
Description	Candida albicans is an opportunist pathogen responsible for a large spectrum of infections, from superficial mycosis to systemic diseases known as candidiasis. C. albicans can grow in various morphological forms, including unicellular budding yeasts, filamentous pseudophyphae and true hyphae, and the ability to switch from yeast to hyphal forms is a key survival mechanism in the adaptation of the pathogen to the microenvironments encountered within the host. The filamentation is regulated by multiple signalling pathways and can be induced, in vitro, in several growth media, often leading to contradictory results in the literature. In this study, we use quantitative proteomic analyses to compare the response of C. albicans yeast cells grown in the minimal medium YNB to four media widely used in the literature to induce the yeast to hyphae transition: YNB-Serum, YNB-N-Acetyl-glucosamine (YNB-NAG), Lee medium and the rich Spider medium. We show here that each growth medium induces a unique pattern of response in C. albicans cells, and that some conditions trigger an original and specific metabolic adaptive response. Moreover, comparison of the proteomic profiles indicates modifications of the thiol-dependent oxidative stress status of the cells, especially in YNB-Serum and Lee medium, and, to a lesser extent, in Spider medium, confirming the role of oxidative stress in the filamentation process. Overall, our data indicate that some hypha-inducing media routinely used in the literature are associated with significant changes in in proteomic signature and that should be more often taken account when exploring the filamentation process of the pathogen.
HostingRepository	PRIDE
AnnounceDate	2024-10-31
AnnouncementXML	Submission_2024-10-31_01:28:25.029.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guillaume CHEVREUX
SpeciesList	scientific name: Candida albicans (Yeast); NCBI TaxID: 5476;
ModificationList	methylthiolated residue; monohydroxylated residue; deamidated residue
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-22 06:21:41	ID requested
⏵ 1	2024-10-31 01:28:25	announced

Dataset with its publication pending

submitter keyword: Candida albicans, metabolism, oxidative stress, proteomic, filamentation

Françoise Auchere
contact affiliation	Université Paris Cité, CNRS, Institut Jacques Monod, 75013 Paris, France
contact email	francoise.auchere@ijm.fr
lab head
Guillaume CHEVREUX
contact affiliation	Institut Jacques Monod, CNRS UMR7592
contact email	guillaume.chevreux@ijm.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD052475
     1. Label: PRIDE project
     2. Name: C. albicans cells exhibit specific proteomic profiles depending on the medium used to induce the filamentation process