PXD052474

PXD052474 is an original dataset announced via ProteomeXchange.

Title	Profiling the Interactome of Folded-State Protein Filaments in Yeast
Description	The formation of large polymeric structures without protein unfolding and aggregation, such as cytoskeletal and enzyme filaments, is crucial for normal cellular function. We term this type of assembly agglomerates. There are several agglomeration-associated diseases, such as sickle cell disease, caused by hemoglobin agglomeration. Nevertheless, it is still unknown whether there is a specific machinery to recognize protein agglomeration or whether it can be recognized by the general proteostasis machinery (e.g., by ubiquitin-dependent degradation). Here, we compared interactomes of wild-type proteins and their mutated counterparts prone to aggregation or agglomeration in yeasts. For this, we performed a pull-down followed by shotgun proteomics to identify proteins that interact with protein aggregates and/or agglomerates. We found that proteostasis machinery (chaperones mainly) actively interacts with protein aggregates, which is not the case for agglomerates. Despite their large size, they are not recognized by proteostasis machinery. Agglomerates remain mainly inert for other protein-protein interactions except for the interaction with several yeast proteins prone to agglomeration per se.
HostingRepository	PRIDE
AnnounceDate	2025-08-21
AnnouncementXML	Submission_2025-08-21_06:10:23.367.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD052474
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Arseniy Lobov
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-22 04:45:54	ID requested
⏵ 1	2025-08-21 06:10:23	announced

10.6019/PXD052474;

submitter keyword: Aggregation, agglomeration, yeasts, proteostasis

Emmanuel Doram Levy
contact affiliation	Dept. of Molecular and Cellular Biology, University of Geneva
contact email	Emmanuel.Levy@unige.ch
lab head
Arseniy Lobov
contact affiliation	Yifat Merbl Lab, Dept. of Systems Immunology, Weizmann Institute of Science
contact email	arseniylobov@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD052474
     1. Label: PRIDE project
     2. Name: Profiling the Interactome of Folded-State Protein Filaments in Yeast