PXD052448

PXD052448 is an original dataset announced via ProteomeXchange.

Title	The Immunopeptidomic Landscape of Ependymomas provide actionable antigens for T cell-based immunotherapy
Description	Ependymoma (EPN) are primary tumors of the nervous system. Due to their growth pattern, most ependymoma can be managed with neurosurgical resection alone. A substantial proportion of these tumors recurs or display infiltrative growth pattern, however. Therapeutic options after neurosurgical resection are limited. Based on tumor board recommendations, radiation therapy will be recommended for some patients. Systemic treatment options for progressive ependymoma include platin-based therapeutic regimes or a combination of lapatinib and temozolomide. Peptide-based immunotherapy represents a promising low-side effect therapeutic option relying on the induction of tumor-specific T cells targeting human leukocyte antigens (HLA)-presented peptides. We here analyzed the landscape of naturally presented HLA class I and II ligands of primary ependymomas using a comparative mass-spectrometry-based immunopeptidomic approach to delineate naturally presented ependymoma-associated antigens. We discovered a subset of EPN-exclusive peptides including HLA-A*02 and HLA-A*25/HLA-A*26–restricted HLA ligands and identified a small panel of cancer/testis antigens (CTAs)-derived HLA ligands. Furthermore, we outlined immunopeptidomic alterations in different EPN-subgroups and progressive EPNs and performed subsequent functional characterization for demonstrating immunogenicity in vitro. Taken together, we provide actionable targets that could further be explored as a T-cell based immunotherapeutic strategy in this tumor entity.
HostingRepository	PRIDE
AnnounceDate	2025-05-26
AnnouncementXML	Submission_2025-05-25_16:35:26.498.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jonas Scheid
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue
Instrument	LTQ Orbitrap XL

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-21 13:58:03	ID requested
⏵ 1	2025-05-25 16:35:27	announced

M, ü, hlenbruch L, Rieger D, Becker H, Santos Leite AM, M, ä, urer I, Schittenhelm J, Dubbelaar M, Bichmann L, Kohlbacher O, Rammensee HG, Gouttefangeas C, Tatagiba M, Walz JS, Tabatabai G, The immunopeptidomic landscape of ependymomas provides actionable antigens for T-cell-based immunotherapy. Neurooncol Adv, 7(1):vdae226(2025) [pubmed]

10.1093/noajnl/vdae226;

submitter keyword: peptide vaccine, HLA ligands, mass spectrometry, cancer vaccination,Neuroepithelial tumor, immunotherapy

Juliane Walz
contact affiliation	1. Department of Peptide-based Immunotherapy, Institute of Immunology, University and University Hospital Tübingen, Germany 2. Cluster of Excellence iFIT (EXC2180) “Image-Guided and Functionally Instructed Tumor Therapies”, University of Tübingen, Germany 3. Clinical Collaboration Unit Translational Immunology, Department of Internal Medicine, University Hospital Tübingen, Germany 4. German Cancer Consortium (DKTK) and German Cancer Research Center (DKFZ), partner site Tübingen, Germany
contact email	juliane.walz@med.uni-tuebingen.de
lab head
Jonas Scheid
contact affiliation	Department of Peptide-based Immunotherapy, University and Universityhospital of Tübingen Quantitative Biology Center, University of Tübingen
contact email	jonas.scheid@uni-tuebingen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD052448

PRIDE project URI

• PRIDE
  1. PXD052448
     1. Label: PRIDE project
     2. Name: The Immunopeptidomic Landscape of Ependymomas provide actionable antigens for T cell-based immunotherapy