PXD052440

PXD052440 is an original dataset announced via ProteomeXchange.

Title	Restriction of Arginine Induces Antibiotic Tolerance in Staphylococcus aureus
Description	Staphylococcus aureus is responsible for a substantial number of invasive infections globally each year. These infections are problematic because they are frequently recalcitrant to antibiotic treatment. Antibiotic tolerance, the ability of bacteria to persist despite normally lethal doses of antibiotics, contributes to antibiotic treatment failure in S. aureus infections. To understand how antibiotic tolerance is induced, S. aureus biofilms exposed to multiple anti-staphylococcal antibiotics were examined using both quantitative proteomics and transposon sequencing. These screens indicated that arginine metabolism is involved in antibiotic tolerance within a biofilm and led to the hypothesis that depletion of arginine within S. aureus communities can induce antibiotic tolerance. Consistent with this hypothesis, inactivation of argH, the final gene in the arginine synthesis pathway, induces antibiotic tolerance. Arginine restriction was found to induce antibiotic tolerance via inhibition of protein synthesis. In murine skin and bone infection models, an argH mutant has enhanced ability to survive antibiotic treatment with vancomycin, highlighting the relationship between arginine metabolism and antibiotic tolerance during S. aureus infection. Uncovering this link between arginine metabolism and antibiotic tolerance has the potential to open new therapeutic avenues targeting previously recalcitrant S. aureus infections.
HostingRepository	PRIDE
AnnounceDate	2024-07-11
AnnouncementXML	Submission_2024-07-11_06:05:08.119.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dale Chaput
SpeciesList	scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-21 06:39:22	ID requested
⏵ 1	2024-07-11 06:05:08	announced

Dataset with its publication pending

submitter keyword: proteomics, Skin and Soft Tissue Infection (SSTI), Biofilm, antibiotic treatment failure, TnSeq, protein synthesis,Staphylococcus aureus

Jeffrey Freiberg
contact affiliation	Instructor in Medicine, Division of Infectious Diseases, Vanderbilt University
contact email	jeffrey.freiberg@vumc.org
lab head
Dale Chaput
contact affiliation	University of South Florida
contact email	chaput@usf.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD052440

PRIDE project URI

• PRIDE
  1. PXD052440
     1. Label: PRIDE project
     2. Name: Restriction of Arginine Induces Antibiotic Tolerance in Staphylococcus aureus